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The aim of this study was to assess the effect of ectopic expression of Oct human amniotic fluid stem cells. We developed a novel method for isolation of putative human amniotic fluid\u2010derived multipotent stem cells. These cells showing mesenchymal stem cell phenotypes (human amniotic fluid\u2010derived mesenchymal stem cells, hAFMSCs) were transfected with a plasmid carrying genes for Oct\u20104 and the green fluorescent protein (GFP). The stably transfected cells, hAFMSCs\u2010Oct4\/GFP, were selected by using G418 and found to express the GFP reporter gene under the control of Oct\u20104 promoter. We found that hAFMSCs developed by our method possess very high self\u2010renewal ability (about 78 cumulative population doublings) and multilineage differentiation potency. Significantly, the hAFMSCs\u2010Oct4\/GFP cells showed enhanced expression of the three major pluripotency genes\n                    <jats:italic>Oct\u20104<\/jats:italic>\n                    ,\n                    <jats:italic>Nanog<\/jats:italic>\n                    , and\n                    <jats:italic>Sox\u20102<\/jats:italic>\n                    , and increased colony\u2010forming ability and growth rate compared with the parental hAFMSCs. We demonstrated that the ectopic expression of\n                    <jats:italic>Oct\u20104<\/jats:italic>\n                    gene in hAFMSCs with high self\u2010renewal ability could upregulate\n                    <jats:italic>Nanog<\/jats:italic>\n                    and\n                    <jats:italic>Sox\u20102<\/jats:italic>\n                    gene expression and enhance cell growth rate and colony\u2010forming efficiency. Therefore, the ectopic expression of Oct\u20104 could be a strategy to develop pluripotency in hAFMSCs for clinical applications.\n                  <\/jats:p>","DOI":"10.1002\/bab.1315","type":"journal-article","created":{"date-parts":[[2014,11,11]],"date-time":"2014-11-11T04:30:47Z","timestamp":1415680247000},"page":"591-597","source":"Crossref","is-referenced-by-count":16,"title":["Upregulation of\n                    <i>Nanog<\/i>\n                    and\n                    <i>Sox\u20102<\/i>\n                    genes following ectopic expression of Oct\u20104 in amniotic fluid mesenchymal stem cells"],"prefix":"10.1002","volume":"62","author":[{"given":"Kai\u2010Hung","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Kuo General Hospital  Tainan Taiwan"},{"name":"Center for Reproductive Medicine Kuo General Hospital  Tainan Taiwan"},{"name":"Department of Laboratory Medicine Kuo General Hospital  Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"An\u2010Pei","family":"Kao","sequence":"additional","affiliation":[{"name":"Department of Research and Development NeoAsia  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia\u2010Cheng","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and Human Development Michigan State University East Lansing  MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ta\u2010Chin","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Kuo General Hospital  Tainan Taiwan"},{"name":"Center for Reproductive Medicine Kuo General Hospital  Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsung\u2010Cheng","family":"Kuo","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Kuo General Hospital  Tainan Taiwan"},{"name":"Center for Reproductive Medicine Kuo General Hospital  Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,5,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deh279"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1274"},{"key":"e_1_2_7_4_1","first-page":"RA253","volume":"8","author":"Prusa A. 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