{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:10:33Z","timestamp":1788869433262,"version":"build-2803163510"},"reference-count":61,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T00:00:00Z","timestamp":1436832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005328","name":"Research Center of the Female Scientific and Medical Colleges","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005328","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In this study, a new strain, ALA1, was identified as\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    by biochemical tests, and its 16S ribosomal DNA sequence was isolated from dromedary milk. ALA1 lipase production was optimized in shake flask experiments and measured with varying pH (3\u201311), temperature (20\u201355\u00a0\u00b0C) and substrate concentrations. The maximum lipase production was recorded at pH 8 and 30\u00a0\u00b0C for up to 30\u00a0H of culture period for the\n                    <jats:italic>S. aureus<\/jats:italic>\n                    ALA1 strain. Among the substrates tested, selected carbon sources, xylose, nitrogen source, yeast extract, and olive oil (1%) were suitable for maximizing lipase production. The effects of surfactants were investigated and showed that Tween 20, Tween 80, and Triton X\u2010100 prevented lipase production. Interestingly, isolate ALA1 was able to grow in high concentrations of benzene or toluene (up to 50% (v\/v)). Moreover, the lipolytic activity of the\n                    <jats:italic>S. aureus<\/jats:italic>\n                    ALA1 lipase was stimulated by diethyl ether, whereas almost 100% of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    ALA1 lipase activity was retained in 25% acetone, acetonitrile, benzene, 2\u2010propanol, ethanol, methanol, or toluene. Because of its stability in organic solvent, the\n                    <jats:italic>S. aureus<\/jats:italic>\n                    ALA1 lipase was used as a biocatalyst to synthesize high levels of added value molecules.\n                    <jats:italic>S. aureus<\/jats:italic>\n                    ALA1 lipase could be considered as an ideal choice for applications in detergent formulations because of its high stability and compatibility with various surfactants, oxidizing agents, and commercial detergents.\n                  <\/jats:p>","DOI":"10.1002\/bab.1381","type":"journal-article","created":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T13:49:25Z","timestamp":1427896165000},"page":"378-390","source":"Crossref","is-referenced-by-count":18,"title":["An organic solvent\u2010stable lipase from a newly isolated\n                    <i>Staphylococcus aureus<\/i>\n                    ALA1 strain with potential for use as an industrial biocatalyst"],"prefix":"10.1002","volume":"63","author":[{"given":"Abir","family":"Ben Bacha","sequence":"first","affiliation":[{"name":"Biochemistry Department, Science College King Saud University  Riyadh Saudi Arabia"},{"name":"Laboratory of Plant Biotechnology Applied to Crop Improvement Faculty of Science of Sfax University of Sfax  Sfax Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadine MS","family":"Moubayed","sequence":"additional","affiliation":[{"name":"Botany and Microbiology Department Science College King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alaa","family":"Al\u2010Assaf","sequence":"additional","affiliation":[{"name":"Biochemistry Department, Science College King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,7,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0141-0229(00)00278-7"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.1994.tb00121.x"},{"key":"e_1_2_8_4_1","author":"BBC Research BIO030F","year":"2011","journal-title":"Enzymes in industrial applications: global markets"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0141-0229(95)00075-5"},{"key":"e_1_2_8_6_1","first-page":"971","volume":"4","author":"Patil K. 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