{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T05:02:50Z","timestamp":1784264570249,"version":"3.55.0"},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,8,26]],"date-time":"2015-08-26T00:00:00Z","timestamp":1440547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005357","name":"APVV","doi-asserted-by":"crossref","award":["SK-SRB-2013-0028"],"award-info":[{"award-number":["SK-SRB-2013-0028"]}],"id":[{"id":"10.13039\/501100005357","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Bilateral cooperation, Ministry of Education, Science and Technological Development of Serbia","award":["451-03-545\/2015-09\/01"],"award-info":[{"award-number":["451-03-545\/2015-09\/01"]}]},{"name":"Ministry of Education, Science and Technological Development of Serbia","award":["173042"],"award-info":[{"award-number":["173042"]}]},{"name":"Slovak Grant Agency for Science VEGA","award":["2\/0162\/12"],"award-info":[{"award-number":["2\/0162\/12"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Glycosylation is co\u2010 and posttranslational modifications affecting proteins. The glycopattern changes are associated with changes in biological function and are involved in many diseases including cancer. We present the lectin\u2010based protein microarray method enabling determination of differences in protein glycosylation. The method involves isolation of targeted protein from samples by immunoprecipitation, spotting of protein from multiple samples into arrays on a microarray slide, incubation with set of biotinylated lectins, the reaction with fluorescent conjugate of streptavidin, and detection of fluorescent intensities by microarray scanner. Lectin\u2010based protein microarray was applied in investigation of differences in alpha\u20102\u2010macroglobulin (\u03b12M) glycosylation isolated from sera samples of healthy persons and patients with colorectal cancer (CC). From 14 lectins used in analysis, statistically significant differences (Student's <jats:italic>t<\/jats:italic>\u2010test, <jats:italic>P<\/jats:italic> &lt; 0.05) between two groups of samples (persons without cancer and CC patients) were found for 5 of them. \u03b12M molecules isolated from sera of CC patients have higher content of \u03b12,6 sialic acid, N\u2010acetylglucosamine and mannose residues, and tri\u2010\/tetraantennary complex type high\u2010mannose N\u2010glycans. A novel lectin\u2010based protein microarray developed and described can serve as a suitable analytical technique for sensitive, simple, fast, and high\u2010throughput determination of differences in protein glycosylation isolated from serum or other samples.<\/jats:p>","DOI":"10.1002\/bab.1407","type":"journal-article","created":{"date-parts":[[2015,6,15]],"date-time":"2015-06-15T09:51:35Z","timestamp":1434361895000},"page":"457-464","source":"Crossref","is-referenced-by-count":24,"title":["Lectin\u2010based protein microarray analysis of differences in serum alpha\u20102\u2010macroglobulin glycosylation between patients with colorectal cancer and persons without cancer"],"prefix":"10.1002","volume":"63","author":[{"given":"Milo\u0161","family":"\u0160underi\u0107","sequence":"first","affiliation":[{"name":"Institute for the Application of Nuclear Energy University of Belgrade  Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alena","family":"\u0160ediv\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Chemistry Slovak Academy of Sciences  Bratislava Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dragana","family":"Robajac","sequence":"additional","affiliation":[{"name":"Institute for the Application of Nuclear Energy University of Belgrade  Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Goran","family":"Milju\u0161","sequence":"additional","affiliation":[{"name":"Institute for the Application of Nuclear Energy University of Belgrade  Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Gemeiner","sequence":"additional","affiliation":[{"name":"Institute of Chemistry Slovak Academy of Sciences  Bratislava Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olgica","family":"Nedi\u0107","sequence":"additional","affiliation":[{"name":"Institute for the Application of Nuclear Energy University of Belgrade  Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaroslav","family":"Katrl\u00edk","sequence":"additional","affiliation":[{"name":"Institute of Chemistry Slovak Academy of Sciences  Bratislava Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,8,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2011.12.001"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.R112.026799"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201300387"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.227"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.20195"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2013.06.009"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2014.07.028"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/cb300262x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1535-6108(03)00086-2"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3cs35419a"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-286-1_15"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr070062p"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10930-013-9471-8"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.4172\/jpb.1000224"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201300376"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00916586"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr500647y"},{"key":"e_1_2_7_19_1","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/j.aca.2014.10.029","volume":"853","author":"Klukova L.","journal-title":"Anal. 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