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The commercial subunit vaccines against PCV2 consist of VLPs formed by the self\u2010assembly of PCV2 capsid protein (CP) expressed in the baculovirus vector system. In this work, a PCV2 protective epitope was inserted into three different regions of papaya ringspot virus (PRSV) CP, namely, the N\u2010 and C\u2010termini and a predicted antigenic region located near the N\u2010terminus. Wild\u2010type and chimeric CPs were modeled <jats:italic>in silico<\/jats:italic>, expressed in <jats:italic>Escherichia coli<\/jats:italic>, purified, and visualized by transmission electron microscopy. This is the first report that shows the formation of chimeric VLPs using PRSV as epitope\u2010presentation scaffold. Moreover, it was found that PCV2 epitope localization strongly influences VLP length. Also, the estimated yields of the chimeric VLPs at a small\u2010scale level ranged between 65 and 80 mg\/L of culture medium. Finally, the three chimeric VLPs induced high levels of immunoglobulin G against the PCV2 epitope in immunized BALB\/c mice, suggesting that these chimeric VLPs can be used for swine immunoprophylaxis against PCV2.<\/jats:p>","DOI":"10.1002\/bab.1491","type":"journal-article","created":{"date-parts":[[2016,3,12]],"date-time":"2016-03-12T20:08:57Z","timestamp":1457813337000},"page":"406-414","source":"Crossref","is-referenced-by-count":9,"title":["Porcine circovirus type 2 protective epitope densely carried by chimeric papaya ringspot virus\u2013like particles expressed in <i>Escherichia coli<\/i> as a cost\u2010effective vaccine manufacture alternative"],"prefix":"10.1002","volume":"64","author":[{"given":"Brenda Eugenia","family":"Aguilera","sequence":"first","affiliation":[{"name":"Unidad de Biotecnolog\u00eda M\u00e9dica y Farmac\u00e9utica Centro de Investigaci\u00f3n y Asistencia en Tecnolog\u00eda y Dise\u00f1o del Estado de Jalisco A.C.  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