{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T11:15:10Z","timestamp":1790075710754,"version":"4.0.1"},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,9,14]],"date-time":"2017-09-14T00:00:00Z","timestamp":1505347200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003093","name":"Ministry of Higher Education, Malaysia","doi-asserted-by":"publisher","award":["311\/CIPPM\/4401005"],"award-info":[{"award-number":["311\/CIPPM\/4401005"]}],"id":[{"id":"10.13039\/501100003093","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003093","name":"Ministry of Higher Education, Malaysia","doi-asserted-by":"publisher","award":["203\/CIPPM\/6711381"],"award-info":[{"award-number":["203\/CIPPM\/6711381"]}],"id":[{"id":"10.13039\/501100003093","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Phage display has been applied successfully as a tool for the generation of monoclonal antibodies (mAbs). Naive antibody libraries are unique as they are able to overcome several limitations associated with conventional mAb generation methods like the hybridoma technology. Here, we performed an <jats:italic>in vitro<\/jats:italic> selection and generation of Fab antibodies against <jats:italic>Brugia malayi<\/jats:italic> SXP protein (<jats:italic>Bm<\/jats:italic>SXP), a recombinant antigen for the detection of lymphatic filariasis. We developed a na\u00efve multi ethnic Fab antibody library with an estimated diversity of 2.99 \u00d7 10<jats:sup>9<\/jats:sup>. The antibody library was used to screen for mAbs against <jats:italic>Bm<\/jats:italic>SXP recombinant antigen. Soluble monoclonal Fab antibodies against <jats:italic>Bm<\/jats:italic>SXP were successfully isolated from the na\u00efve library. The Fab antibodies obtained were expressed and analyzed to show its binding capability. The diversity obtained from a pool of donors from various ethnic groups allowed for a diverse antibody library to be generated. The mAbs obtained were also functional in soluble form, which makes it useful for further downstream applications. We believe that the Fab mAbs are valuable for further studies and could also contribute to improvements in the diagnosis of filariasis.<\/jats:p>","DOI":"10.1002\/bab.1591","type":"journal-article","created":{"date-parts":[[2017,8,21]],"date-time":"2017-08-21T14:52:35Z","timestamp":1503327155000},"page":"346-354","source":"Crossref","is-referenced-by-count":13,"title":["Generation and selection of na\u00efve Fab library for parasitic antigen: Anti\u2010<i>Bm<\/i>SXP antibodies for lymphatic filariasis"],"prefix":"10.1002","volume":"65","author":[{"given":"Noorsharmimi","family":"Omar","sequence":"first","affiliation":[{"name":"Institute for Research in Molecular Medicine Universiti Sains Malaysia  Minden Penang Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nurul Hamizah","family":"Hamidon","sequence":"additional","affiliation":[{"name":"Institute for Research in Molecular Medicine Universiti Sains Malaysia  Minden Penang Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Hafiznur","family":"Yunus","sequence":"additional","affiliation":[{"name":"Institute for Research in Molecular Medicine Universiti Sains Malaysia  Minden Penang Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahmah","family":"Noordin","sequence":"additional","affiliation":[{"name":"Institute for Research in Molecular Medicine Universiti Sains Malaysia  Minden Penang Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yee Siew","family":"Choong","sequence":"additional","affiliation":[{"name":"Institute for Research in Molecular Medicine Universiti Sains Malaysia  Minden Penang Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0656-3045","authenticated-orcid":false,"given":"Theam Soon","family":"Lim","sequence":"additional","affiliation":[{"name":"Institute for Research in Molecular Medicine Universiti Sains Malaysia  Minden Penang Malaysia"},{"name":"Analytical Biochemistry Research Centre Universiti Sains Malaysia  Penang Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,9,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-554-1_6"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.26.18218"},{"key":"e_1_2_8_4_1","first-page":"391","volume":"97","author":"Smith G. 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