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In the present study, an aqueous two\u2010phase micellar system (ATPMS) was evaluated to extract human adenovirus type 5 particles from a cell lysate. Adenovirus was cultured in human embryonic kidney 293 (HEK\u2010293) cells to a concentration of 1.4 \u00d7 10<jats:sup>10<\/jats:sup> particles\/mL. Cells were lysed, and the system formed by direct addition of Triton X\u2010114 in a 2<jats:sup>3<\/jats:sup> full factorial design with center points. The systems were formed with Triton X\u2010114 at a final concentration of 1.0, 6.0, and 11.0% (w\/w), cell lysate pH of 6.0, 6.5, and 7.0, and incubation temperatures at 33, 35, and 37\u00a0\u00b0C. Adenovirus particles recovered from partition phases were measured by qPCR. The best system condition was with 11.0% (w\/w) of Triton X\u2010114, a cell lysate pH of 7.0, and an incubation temperature at 33\u00a0\u00b0C, yielding 3.51 \u00d7 10<jats:sup>10<\/jats:sup> adenovirus particles\/mL, which increased the initial adenovirus particles concentration by 2.3\u2010fold, purifying it by 2.2\u2010fold from the cell lysate, and removing cell debris. In conclusion, these results demonstrated that the use of an aqueous two\u2010phase micellar system in the early steps of downstream processing could improve viral particle extraction from cultured cells while integrating clarification, concentration, and prepurification steps.<\/jats:p>","DOI":"10.1002\/bab.1627","type":"journal-article","created":{"date-parts":[[2017,12,5]],"date-time":"2017-12-05T11:49:33Z","timestamp":1512474573000},"page":"381-389","source":"Crossref","is-referenced-by-count":6,"title":["Application of aqueous two\u2010phase micellar system to improve extraction of adenoviral particles from cell lysate"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2475-9807","authenticated-orcid":false,"given":"Jo\u00e3o Vitor Dutra","family":"Molino","sequence":"first","affiliation":[{"name":"Department of Biochemical and Pharmaceutical Technology School of Pharmaceutical Sciences University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9 Moreni","family":"Lopes","sequence":"additional","affiliation":[{"name":"Department of Bioprocess and Biotechnology School of Pharmaceutical Sciences S\u00e3o Paulo State University  Araraquara Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela de Ara\u00fajo","family":"Viana Marques","sequence":"additional","affiliation":[{"name":"University of Pernambuco Campus Serra Talhada  Serra Talhada Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priscila Gava","family":"Mazzola","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences University of Campinas  Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joas Lucas","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Clinical and Toxicological Analysis School of Pharmaceutical Sciences University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario Hiroyuki","family":"Hirata","sequence":"additional","affiliation":[{"name":"Department of Clinical and Toxicological Analysis School of Pharmaceutical Sciences University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ros\u00e1rio Dominguez Crespo","family":"Hirata","sequence":"additional","affiliation":[{"name":"Department of Clinical and Toxicological Analysis School of Pharmaceutical Sciences University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Silvia Viccari","family":"Gatti","sequence":"additional","affiliation":[{"name":"Department of Genetics Evolution and Bioagents Institute of Biology University of Campinas  Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adalberto","family":"Pessoa","sequence":"additional","affiliation":[{"name":"Department of Biochemical and Pharmaceutical Technology School of Pharmaceutical Sciences University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,22]]},"reference":[{"key":"e_1_2_6_2_1","unstructured":"Vectors used in gene therapy clinical trials. 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