{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T01:24:29Z","timestamp":1793409869418,"version":"4.5.0"},"reference-count":53,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,12,15]],"date-time":"2017-12-15T00:00:00Z","timestamp":1513296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Royal Golden Jubilee Ph.D. program","award":["PhD 0258\/2552"],"award-info":[{"award-number":["PhD 0258\/2552"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Novel antioxidant and anti\u2010inflammatory peptides were isolated from hydrolysates of Siamese crocodile (\n                    <jats:italic>Crocodylus siamensis<\/jats:italic>\n                    ) hemoglobin.\n                    <jats:italic>C. siamensis<\/jats:italic>\n                    hemoglobin hydrolysates (CHHs) were obtained by pepsin digestion at different incubation times (2, 4, 6, and 8 H) at 37\u00a0\u00b0C and subjected to antioxidant and anti\u2010inflammatory activity assessment. CHH obtained by 2\u2010H hydrolysis (2H\u2010CHH) showed the highest anti\u2010inflammatory activity with respect to decreasing nitric oxide (NO) production, whereas the strongest antioxidant activity was found for 6\u2010H hydrolysis (6H\u2010CHH) against nitric oxide radicals. To evaluate the anti\u2010inflammatory and antioxidant activity of individual peptide components, 2H\u2010CHH and 6H\u2010CHH were purified by semipreparative HPLC. Peptide fraction P57 isolated from 6H\u2010CHH was found to exhibit the highest nitric oxide radical inhibition activity (32.0%). Moreover, purification of 2H\u2010CHH yielded peptide fraction P16, which displayed a high efficacy in decreasing NO production of macrophage RAW 264.7 cells (83.2%) and significantly reduced proinflammatory cytokines and inflammatory mediators interleukin\u20106 (IL\u20106), interleukin\u20101 beta (IL\u20101\u03b2), and prostaglandin\u2010E2 (PGE\n                    <jats:sub>2<\/jats:sub>\n                    ) production to about 2.0, 0.3, and 1.9 ng\/mL, respectively. Using LTQ orbitrap XL mass spectrometry, active peptide sequences were identified as antioxidant KIYFPHF (KF7), anti\u2010inflammatory SAFNPHEKQ (SQ9), and IIHNEKVQAHGKKVL (IL15). Additionally, CHHs simulated gastric and intestinal\n                    <jats:italic>in vitro<\/jats:italic>\n                    digestion positively contributed to antioxidant and anti\u2010inflammatory activity. Taken collectively, the results of this work demonstrate that CHHs contain several peptides with anti\u2010inflammatory and antioxidant properties, which may prove valuable as treatment or supplement against diseases associated with inflammation and oxidative stress.\n                  <\/jats:p>","DOI":"10.1002\/bab.1628","type":"journal-article","created":{"date-parts":[[2017,11,22]],"date-time":"2017-11-22T02:52:47Z","timestamp":1511319167000},"page":"455-466","source":"Crossref","is-referenced-by-count":23,"title":["Novel antioxidant and anti\u2010inflammatory peptides from the Siamese crocodile (\n                    <i>Crocodylus siamensis<\/i>\n                    ) hemoglobin hydrolysate"],"prefix":"10.1002","volume":"65","author":[{"given":"Jiraporn","family":"Lueangsakulthai","sequence":"first","affiliation":[{"name":"Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science Khon Kaen University Khon Kaen Thailand"},{"name":"Department of Biochemistry, Faculty of Science Khon Kaen University Khon Kaen Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santi","family":"Phosri","sequence":"additional","affiliation":[{"name":"Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science Khon Kaen University Khon Kaen Thailand"},{"name":"Office of Education, Faculty of Engineering Burapha University Chonburi Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tinnakorn","family":"Theansungnoen","sequence":"additional","affiliation":[{"name":"Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science Khon Kaen University Khon Kaen Thailand"},{"name":"Department of Biochemistry, Faculty of Science Khon Kaen University Khon Kaen Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nisachon","family":"Jangpromma","sequence":"additional","affiliation":[{"name":"Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science Khon Kaen University Khon Kaen Thailand"},{"name":"Office of the Dean, Faculty of Science Khon Kaen University Khon Kaen Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theeranan","family":"Temsiripong","sequence":"additional","affiliation":[{"name":"Srirachamoda Co., Ltd. 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