{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T04:19:47Z","timestamp":1775881187659,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,2,7]],"date-time":"2018-02-07T00:00:00Z","timestamp":1517961600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81360544"],"award-info":[{"award-number":["81360544"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81560763"],"award-info":[{"award-number":["81560763"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Interstitial cells of Cajal (ICC), especially myenteric interstitial cells of Cajal (ICC\u2010MY), are key to gastrointestinal motility. However, their role in the pathogenesis of functional dyspepsia (FD) is unclear. Therefore, autophagy and differentiation of ICC\u2010MY were investigated to elucidate the pathogenesis of gastric motility disorder in FD. FD model was induced by chronic stress via tail clamping in rats, which was assessed by the vital signs of rats, gastric emptying rate result, and histology. The ultrastructure of ICC\u2010MY was examined using transmission electron microscope. In ICC\u2010MY, changes in autophagic biomarkers (Beclin1 and LC3B) and differentiation biomarkers (c\u2010kit and SCF) were evaluated with <jats:italic>in situ<\/jats:italic> hybridization, quantitative real time PCR, immunofluorescence, and Western blot, respectively. The FD model was successfully induced in rats, as evidenced by the abnormal vital signs (such as loss of appetite, liquid excreta, less activity, and slower weight gain), the decrease in gastric emptying rates, and little pathological change in gastric antrum tissue. Compared with the control group, FD caused increased organelle denaturation or reduction and increase in vacuolization. FD also promoted generation of autophagosomes in ICC\u2010MY. Moreover, increased the expression of Beclin1 and LC3B, but decreased expression of c\u2010kit and SCF. Excessive autophagy and abnormal differentiation of ICC\u2010MY may contribute to the pathogenesis of gastric motility disorder in FD.<\/jats:p>","DOI":"10.1002\/bab.1635","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T10:31:17Z","timestamp":1514111477000},"page":"533-539","source":"Crossref","is-referenced-by-count":32,"title":["Investigation of autophagy and differentiation of myenteric interstitial cells of Cajal in the pathogenesis of gastric motility disorders in rats with functional dyspepsia"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1671-4716","authenticated-orcid":false,"given":"Li\u2010min","family":"Zhang","sequence":"first","affiliation":[{"name":"The First Affiliated Hospital of Guangxi Medical University  Nanning People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li\u2010jun","family":"Zeng","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Guangxi Medical University  Nanning People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Deng","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Guangxi Medical University  Nanning People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010qin","family":"Zhang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Guangxi Medical University  Nanning People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010jiao","family":"Wang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Guangxi Medical University  Nanning People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tian\u2010yi","family":"Xie","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Guangxi Medical University  Nanning People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiang\u2010hong","family":"Ling","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Guangxi Medical University  Nanning People's Republic of China"},{"name":"The Affiliated Zhoupu Hospital of Shanghai Health Medical College  Shanghai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,2,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.1996.v111.pm8690216"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1254\/jphs.CRJ04002X"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2002.05851.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1746.2002.02756.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-200302000-00012"},{"key":"e_1_2_7_7_1","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1097\/MCG.0b013e3181bc9ebf","volume":"44","author":"Villanacci V.","year":"2010","journal-title":"J. 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