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In the current work, we examined the effect of pulsed laser in the continuous laboratory\u2010scale process on cell disruption. Design\u2010of\u2010experiments (DOE) methodology was used for optimization of cell disruption process to obtain the highest protein concentration in the disruption buffer. Our investigations for the pulsed laser at wavelength of 1,064\u00a0nm demonstrated that for disrupting <jats:italic>P. pastoris<\/jats:italic> cell and releasing rHBsAg, the laser power was the most influential factor, and laser pulse duration and cycle number were in the second and third places. According to the results, the effect of laser power and pulse duration (time) had a direct relationship with protein concentration. For the number of cycles, however, increasing the value from the lowest point at first led to the enhancement and then reduction of protein concentration. The maximum cell disruption and rHBsAg release were recorded for the laser system in the energy input of 284\u00a0mW and the pulse duration of 100\u00a0mSec after four complete rounds of circulation.<\/jats:p>","DOI":"10.1002\/bab.1701","type":"journal-article","created":{"date-parts":[[2018,10,15]],"date-time":"2018-10-15T04:59:56Z","timestamp":1539579596000},"page":"91-100","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["High recovery of intracellular recombinant HBsAg from <i>Pichia pastoris<\/i> via continuous pulsed laser cell disruption system optimized by response surface methodology"],"prefix":"10.1002","volume":"66","author":[{"given":"Maryam","family":"Gazor","sequence":"first","affiliation":[{"name":"Production and Research Complex, Department of Recombinant Products Pasteur Institute of Iran  Tehran Iran"},{"name":"Department of Chemical Engineering, Faculty of Engineering University of Guilan  Rasht Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seyed Siamak","family":"Ashraf Talesh","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Faculty of Engineering University of Guilan  Rasht Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1457-0974","authenticated-orcid":false,"given":"Seyed Nezamedin","family":"Hosseini","sequence":"additional","affiliation":[{"name":"Production and Research Complex, Department of Recombinant Products Pasteur Institute of Iran  Tehran Iran"},{"name":"Viral Vaccines Research Center Pasteur Institute of Iran  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5146-4698","authenticated-orcid":false,"given":"Amin","family":"Javidanbardan","sequence":"additional","affiliation":[{"name":"Production and Research Complex, Department of Recombinant Products Pasteur Institute of Iran  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryam","family":"Khatami","sequence":"additional","affiliation":[{"name":"Production and Research Complex, Department of Recombinant Products Pasteur Institute of Iran  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1385\/MB:16:1:23"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02942184"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-014-5732-5"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2015.05.008"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2018.06.043"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2013.09.030"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2859-10-48"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/bab.1578"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/abio.370130207"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1656(99)00201-1"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules191220941"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2009.04.004"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12223-011-0069-2"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12257-017-0261-6"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0734-9750(95)02007-P"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbt.2011.09.005"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12257-008-0020-9"},{"key":"e_1_2_7_19_1","first-page":"592","volume":"15","author":"Yusaf T.","year":"2015","journal-title":"Ann. 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