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To mitigate cecB2's toxic inhibition of host cells, various cecB2 constructs were built. Results showed that the combination of a chitin\u2010binding domain and an intein self\u2010cleavage motif in front of cecropin B2, without a His\u2010tag, was best for cecB2 expression. <jats:italic>E.\u00a0coli<\/jats:italic> ER2566 was the best host, and 2YT was the best medium for cultivation. Under these conditions, a cecB2 yield of 98.2\u00a0mg\/L could be obtained after purification. The purified cecB2 expressed a wide antimicrobial effect on most Gram\u2010negative strains, including multidrug\u2010resistant <jats:italic>Acinetobactor baumannii<\/jats:italic> and <jats:italic>Staphylococcus aureus<\/jats:italic>. 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