{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T11:56:59Z","timestamp":1777463819707,"version":"3.51.4"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,5,30]],"date-time":"2019-05-30T00:00:00Z","timestamp":1559174400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Canine parvovirus type 2 (CPV\u20102), as a highly contagious and potentially fatal disease of dogs and many other carnivores, usually causes severe gastroenteritis and myocarditis. Therefore, it is very necessary and urgent to have an accurate method to determine the CPV\u20102 antibodies (CPV\u20102\u2010Ab) in canine samples. Here, a magnetic bead\u2013based chemiluminescence immunoassay was established and optimized to detect the concentration of CPV\u20102\u2010Ab in serum. And a commercial assay was also used to evaluate the consistency with our method. After optimization of the detective system, the CPV\u20102\u2010Ab was captured by CPV\u2010antigen\u2010magnetic bead (8.3\u00a0\u00b5g\/mL); then combined with the conjugation of anti\u2010canine IgG antibody\u2010acridinium ester (0.36\u00a0\u00b5g\/mL). Finally, collected the signal (read the luminosity) after 1 H reaction time. The linear correlation coefficient (<jats:italic>R<jats:sup>2<\/jats:sup><\/jats:italic>) is 0.9924. The limit of detection (sensitivity) is 0.36\u00a0ng\/mL (the linear dynamic range: 1.32\u201393.75\u00a0ng\/mL), and the average recovery is 100.89% without cross\u2010reactions with other canine viral antibodies. The results\u2019 correlation between commercial assays and this method is 0.9888. This immunoassay establishes that it has high sensitivity, accuracy, and specificity in clinical analysis, indicating that this method could be suitable for quantitative detection of CPV\u20102\u2010Ab and evaluation of vaccination effect.<\/jats:p>","DOI":"10.1002\/bab.1758","type":"journal-article","created":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T03:26:33Z","timestamp":1556681193000},"page":"586-590","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Determination of parvovirus antibodies in canine serum using magnetic bead\u2010based chemiluminescence immunoassay"],"prefix":"10.1002","volume":"66","author":[{"given":"Cuicui","family":"Chen","sequence":"first","affiliation":[{"name":"Guangzhou Youdi Bio\u2010technology Co., Ltd.  Guangzhou 510663 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoxiao","family":"Guo","sequence":"additional","affiliation":[{"name":"Guangzhou Youdi Bio\u2010technology Co., Ltd.  Guangzhou 510663 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huankun","family":"Liang","sequence":"additional","affiliation":[{"name":"Guangzhou Youdi Bio\u2010technology Co., Ltd.  Guangzhou 510663 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Ning","sequence":"additional","affiliation":[{"name":"Guangzhou Youdi Bio\u2010technology Co., Ltd.  Guangzhou 510663 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiexing","family":"Li","sequence":"additional","affiliation":[{"name":"Guangzhou Youdi Bio\u2010technology Co., Ltd.  Guangzhou 510663 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuhai","family":"Zhong","sequence":"additional","affiliation":[{"name":"Guangzhou Youdi Bio\u2010technology Co., Ltd.  Guangzhou 510663 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xipan","family":"Liu","sequence":"additional","affiliation":[{"name":"Guangzhou Youdi Bio\u2010technology Co., Ltd.  Guangzhou 510663 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1663-4142","authenticated-orcid":false,"given":"Laiqing","family":"Li","sequence":"additional","affiliation":[{"name":"Guangzhou Youdi Bio\u2010technology Co., Ltd.  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