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Hydrogen sulfide was found to be involved in GCs\u2010induced osteoblast dysfunction. Osteoblastic MC3T3\u2010E1 cell and mitochondrial function were determined by cell viability, M\u2010CSF level, and ALP activity and superoxide production, membrane potential, and ATP level, respectively. The purpose of this research was to explore the impact of NaHS on osteoblastic MC3T3\u2010E1 cell function as well as on Sirt1 and PGC1<jats:italic>\u03b1<\/jats:italic> expression in dexamethasone (DEX)\u2010treated osteoblast cells. DEX\u2010treated MC3T3\u2010E1 cells exhibited decreased cell viability and ALP activity, as well as increased M\u2010CSF level; all these changes were dramatically attenuated by NaHS. DEX\u2010treated cells also displayed mitochondrial dysfunction, namely decreased mitochondrial membrane potential and ATP generation and increased superoxide generation, which were partly reversed by NaHS. We confirmed decreased Sirt1 and PGC1<jats:italic>\u03b1<\/jats:italic> protein expression in DEX\u2010treated MC3T3\u2010E1 cells by Western blot, which was also partly reversed by NaHS. Silencing of Sirt1 abrogated the protective effect of NaHS against DEX\u2010induced cell damage and mitochondrial dysfunction. NaHS alleviates DEX\u2010induced osteoblastic MC3T3\u2010E1 cell injury by improving mitochondrial function.<\/jats:p>","DOI":"10.1002\/bab.1786","type":"journal-article","created":{"date-parts":[[2019,6,7]],"date-time":"2019-06-07T11:38:28Z","timestamp":1559907508000},"page":"690-697","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Sodium hydrosulfide mitigates dexamethasone\u2010induced osteoblast dysfunction by interfering with mitochondrial function"],"prefix":"10.1002","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1796-8283","authenticated-orcid":false,"given":"Jun","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Orthopedic Trauma Surgery Changzheng Hospital, The Second Military Medical University  Huangpu District Shanghai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Trauma Surgery Changzheng Hospital, The Second Military Medical University  Huangpu District Shanghai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Trauma and Orthopedics YueYang Hospital  Shanghai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Trauma Surgery Changzheng Hospital, The Second Military Medical University  Huangpu District Shanghai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suchi","family":"Qiao","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Trauma Surgery Changzheng Hospital, The Second Military Medical University  Huangpu District Shanghai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Trauma and Orthopedics Peking University People's Hospital  Beijing People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aimin","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Trauma Surgery Changzheng Hospital, The Second Military Medical University  Huangpu District Shanghai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,6,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)62349-5"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2017.03.021"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00268-006-0143-0"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3164\/jcbn.10-58"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2016.3179"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.5071"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2015.6596"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-015-9212-4"},{"key":"e_1_2_8_10_1","first-page":"5501","volume":"172","author":"Meng G.","year":"2015","journal-title":"J. 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