{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T10:44:43Z","timestamp":1780051483058,"version":"3.53.1"},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,12,5]],"date-time":"2019-12-05T00:00:00Z","timestamp":1575504000000},"content-version":"vor","delay-in-days":34,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81560186"],"award-info":[{"award-number":["81560186"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Chronic bacterial infections in the oral cavity influence the development of dental caries. Mutans streptococci are the major pathogenic cause of dental caries. The World Health Organization (WHO) ranks dental caries, cancer, and cardiovascular diseases as the three major global diseases that need urgent preventative and curative measures. However, substantial evidence suggests that traditional prevention and treatment strategies are inefficient in reducing the prevalence of dental caries. For protection against caries, it is important to develop effective vaccines that induce anticolonizing immunity against <jats:italic>Streptococcus mutans<\/jats:italic> infections. In the present investigation, we constructed a fusion anti\u2010caries DNA vaccine (PAcA\u2010ctxB) through fusing A region of cell surface protein PAc (PAcA) coding gene of mutans streptococci with cholera toxin B subunit coding gene (CTB). Afterward, the plasmids were integrated into tomato genomes through agrobacterium\u2010mediated plant transformation technology. The presence of transgenes in the tomato genome was confirmed by PCR, \u03b2\u2010glucuronidase gene (GUS), and western blot. The expression of genes was confirmed at transcription and protein level. Altogether, the results presented herein showed that transgenic tomatoes may provide a useful system for the production of human caries antigen.<\/jats:p>","DOI":"10.1002\/bab.1806","type":"journal-article","created":{"date-parts":[[2019,8,21]],"date-time":"2019-08-21T22:48:32Z","timestamp":1566427712000},"page":"924-929","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Construction of a fusion anti\u2010caries DNA vaccine in transgenic tomato plants for <i>PAcA<\/i> gene and cholera toxin B subunit"],"prefix":"10.1002","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8095-0633","authenticated-orcid":false,"given":"Guohui","family":"Bai","sequence":"first","affiliation":[{"name":"Special Key Laboratory of Oral Diseases Research, Higher Education Institution in Guizhou Province Zunyi Medical University  Zunyi People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Tian","sequence":"additional","affiliation":[{"name":"Special Key Laboratory of Oral Diseases Research, Higher Education Institution in Guizhou Province Zunyi Medical University  Zunyi People's Republic of China"},{"name":"Hospital of Stomatology Zunyi Medical University  Zunyi People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayuan","family":"Wu","sequence":"additional","affiliation":[{"name":"Special Key Laboratory of Oral Diseases Research, Higher Education Institution in Guizhou Province Zunyi Medical University  Zunyi People's Republic of China"},{"name":"Hospital of Stomatology Zunyi Medical University  Zunyi People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Gu","sequence":"additional","affiliation":[{"name":"Special Key Laboratory of Oral Diseases Research, Higher Education Institution in Guizhou Province Zunyi Medical University  Zunyi People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhu","family":"Chen","sequence":"additional","affiliation":[{"name":"Guiyang Hospital of Stomatology  Guiyang People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengjiao","family":"Zeng","sequence":"additional","affiliation":[{"name":"Special Key Laboratory of Oral Diseases Research, Higher Education Institution in Guizhou Province Zunyi Medical University  Zunyi People's Republic of China"},{"name":"Hospital of Stomatology Zunyi Medical University  Zunyi People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianguo","family":"Liu","sequence":"additional","affiliation":[{"name":"Special Key Laboratory of Oral Diseases Research, Higher Education Institution in Guizhou Province Zunyi Medical University  Zunyi People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,12,5]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"71","volume":"22","author":"Petersen P. 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