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Human interferon\u2010gamma (hIFN\u2010\u03b3), a type II cytokines, are having potent antiproliferative and immunomodulatory effects. In the current study, the fed\u2010batch cultivation of recombinant<jats:italic>Pichia pastoris<\/jats:italic>was carried out, and its effect on cell biomass production, recombinant human interferon\u2010gamma (rhIFN\u2010\u03b3) production, and the overflow metabolites was estimated.<jats:italic>P. pastoris<\/jats:italic>GS115 strain coexpressed with 6\u2010phosphogluconolactonase (SOL3) and ribulose\u2010phosphate 3\u2010epimerase (RPE1) gene (GS115\/rhIFN\u2010\u03b3\/SR) resulted in 60\u00a0mg\u00a0L<jats:sup>\u22121<\/jats:sup>of rhIFN\u2010\u03b3 production, which was twofold higher as compared with the production from GS115\/rhIFN\u2010\u03b3 strain. The antiproliferative potential of rhIFN\u2010\u03b3 was examined on the human squamous carcinoma (A431) cell lines. Cells treated with 80\u00a0ng\u00a0mL<jats:sup>\u22121<\/jats:sup>of rhIFN\u2010\u03b3 exhibited 50% growth inhibition by enhancing the production of intracellular reactive oxygen species levels and disrupting membrane integrity. Our findings highlight a state of art process development strategy for the high\u2010level production of rhIFN\u2010\u03b3 and its potential application as a therapeutic drug in SCC therapy.<\/jats:p>","DOI":"10.1002\/bab.1868","type":"journal-article","created":{"date-parts":[[2019,12,7]],"date-time":"2019-12-07T06:11:55Z","timestamp":1575699115000},"page":"973-982","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Glucose\u2013methanol\u2010based fed\u2010batch fermentation for the production of recombinant human interferon gamma (rhIFN\u2010\u03b3) and evaluation of its antitumor potential"],"prefix":"10.1002","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8098-7869","authenticated-orcid":false,"given":"Ashish A","family":"Prabhu","sequence":"first","affiliation":[{"name":"Biochemical Engineering Laboratory Department of Biosciences and Bioengineering Indian Institute of Technology Guwahati (IITG) Guwahati Assam India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jadi Praveen","family":"Kumar","sequence":"additional","affiliation":[{"name":"Biomaterial and Tissue Engineering Laboratory Department of Biosciences and Bioengineering Indian Institute of Technology Guwahati (IITG) Guwahati Assam India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3936-4621","authenticated-orcid":false,"given":"Biman B.","family":"Mandal","sequence":"additional","affiliation":[{"name":"Biomaterial and Tissue Engineering Laboratory Department of Biosciences and Bioengineering Indian Institute of Technology Guwahati (IITG) Guwahati Assam India"},{"name":"Centre for Nanotechnology Indian Institute of Technology Guwahati Guwahati Assam India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6977-4915","authenticated-orcid":false,"given":"Venkata Dasu","family":"Veeranki","sequence":"additional","affiliation":[{"name":"Biochemical Engineering Laboratory Department of Biosciences and Bioengineering Indian Institute of Technology Guwahati (IITG) Guwahati Assam India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,2,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/cgt.2011.36"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2009.151"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2003.11.007"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(08)70178-5"},{"key":"e_1_2_9_6_1","first-page":"3174","volume":"156","author":"Min W.","year":"1996","journal-title":"J. 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