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The objective in this work was to evaluate possible <jats:italic>in vitro<\/jats:italic> interactions between vanillic acid and enzymes from <jats:italic>Bothrops<\/jats:italic> spp. and <jats:italic>Crotalus durissus terrificus<\/jats:italic> venoms, and also suggest a theory as how they interact based on molecular docking. Vanillic acid inhibited the phospholipase activity induced by <jats:italic>Bothrops alternatus<\/jats:italic> (\u223c25% inhibition); the caseinolytic activity induced by <jats:italic>Bothrops atrox<\/jats:italic> (\u223c30%), <jats:italic>Bothrops jararacussu<\/jats:italic> (\u223c44%), and <jats:italic>C. d. terrificus<\/jats:italic> (\u223c33%); the fibrinogenolysis induced by <jats:italic>B. jararacussu<\/jats:italic>, <jats:italic>B. atrox<\/jats:italic>, and <jats:italic>C. d. terrificus<\/jats:italic> (100%); the serine protease activity induced by <jats:italic>Bothrops moojeni<\/jats:italic> (\u223c45%) and <jats:italic>Bothrops jararaca<\/jats:italic> (\u223c66%); the hemolytic activity induced by <jats:italic>B. moojeni<\/jats:italic> (\u223c26%); the thrombolysis activity induced by <jats:italic>B. atrox<\/jats:italic> (\u223c30%) and <jats:italic>B. jararacussu<\/jats:italic> (\u223c20%); and the thrombotic activity induced by <jats:italic>C. d. terrificus<\/jats:italic> (\u223c8%). The compound was also capable of delaying the coagulation time in citrated plasma by 60, 35, and 75 Sec, when incubated with <jats:italic>B. moojeni<\/jats:italic>, <jats:italic>B. atrox<\/jats:italic>, and <jats:italic>B. jararaca<\/jats:italic>, respectively. The results obtained expand the possibilities for future pharmaceutical use of vanillic acid, considering the high homology degree among human and snake venom phospholipases A<jats:sub>2<\/jats:sub> and proteases (involved in chronic inflammatory diseases). Also, this compound can be used as adjuvant to improve currently available treatments for ophidism victims.<\/jats:p>","DOI":"10.1002\/bab.1943","type":"journal-article","created":{"date-parts":[[2020,5,18]],"date-time":"2020-05-18T12:23:14Z","timestamp":1589804594000},"page":"486-496","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Vanillic acid as phospholipase A<sub>2<\/sub> and proteases inhibitor: <i>In vitro<\/i> and computational analyses"],"prefix":"10.1002","volume":"68","author":[{"given":"Pedro H.","family":"S. 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