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The traditional process of virus purification is time\u2010consuming, laborious and hard to scale up. Here, a rapid, simple and extensible laboratory program for the purification of Japanese encephalitis virus (JEV) was developed by using differential centrifugation, ultrafiltration, Sepharose 4 fast flow gel chromatography, and Capto<jats:sup>TM<\/jats:sup> Core 700 chromatography. The entire process recovered 61.64% of the original virus, and the purified virus particles maintained good activity and immunogenicity. The purification process described has potential application in large\u2010scale production of high\u2010purity JEV.<\/jats:p>","DOI":"10.1002\/bab.1960","type":"journal-article","created":{"date-parts":[[2020,5,27]],"date-time":"2020-05-27T07:24:51Z","timestamp":1590564291000},"page":"547-553","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Purification of cell\u2010derived Japanese encephalitis virus by dual\u2010mode chromatography"],"prefix":"10.1002","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6440-8812","authenticated-orcid":false,"given":"Fuliang","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Northwest A&amp;F University  Yangling People's Republic of China"},{"name":"College of Biology and Food Engineering Anyang Institute of Technology  Anyang People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Luo","sequence":"additional","affiliation":[{"name":"Henan Provincial Key Laboratory of Animal Immunology Key Laboratory of Animal Immunology of the Ministry of Agriculture Henan Academy of Agriculture Sciences  Zhengzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Man","family":"Teng","sequence":"additional","affiliation":[{"name":"Henan Provincial Key Laboratory of Animal Immunology Key Laboratory of Animal Immunology of the Ministry of Agriculture Henan Academy of Agriculture Sciences  Zhengzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangxu","family":"Xing","sequence":"additional","affiliation":[{"name":"Henan Provincial Key Laboratory of Animal Immunology Key Laboratory of Animal Immunology of the Ministry of Agriculture Henan Academy of Agriculture Sciences  Zhengzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junqing","family":"Guo","sequence":"additional","affiliation":[{"name":"Henan Provincial Key Laboratory of Animal Immunology Key Laboratory of Animal Immunology of the Ministry of Agriculture Henan Academy of Agriculture Sciences  Zhengzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yihua","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Northwest A&amp;F University  Yangling People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,6,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.10.085233"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1501.080311"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/09602010600989620"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-3527(03)59002-9"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.44.100190.003245"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1067"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(87)90144-9"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-40-3-531"},{"key":"e_1_2_7_10_1","first-page":"431","volume":"12","author":"Melvin M. 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