{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:51:09Z","timestamp":1777942269962,"version":"3.51.4"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T00:00:00Z","timestamp":1594684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100009334","name":"Pearl River S and T Nova Program of Guangzhou","doi-asserted-by":"publisher","award":["201906010055"],"award-info":[{"award-number":["201906010055"]}],"id":[{"id":"10.13039\/501100009334","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>As a highly contagious and potentially fatal disease of dogs, canine parvovirus type 2 (CPV\u20102) usually causes severe myocarditis and gastroenteritis, while vaccine injection has greatly reduced the incidence of CPV\u20102 diseases. However, there is currently a lack of simple and effective method for quantitative detection of CPV\u20102 in vaccine. Therefore, this study aims to prepare an accurate method to determine the CPV\u20102 antigen (CPV\u20102\u2010Ag) in vaccine. Here, a sandwich time\u2010resolved fluorescence immunoassay (TRFIA) was established and optimized. Anti\u2010CPV\u20102 antibodies were immobilized on 96\u2010well plates to capture CPV\u20102\u2010Ag, and then bound together with the detection antibodies labeled with Europium(III) (Eu<jats:sup>3+<\/jats:sup>) chelates; finally, time\u2010resolved fluorometry was employed to measure the fluorescence intensity. Vaccination was performed to evaluate the relationship between CPV\u20102\u2010Ag concentration and antibody titer. The sensitivity is 1.15\u00a0mEU\/mL (Log<jats:italic>Y<\/jats:italic>\u00a0=\u00a01.524\u00a0+\u00a00.8667\u00a0\u00d7\u00a0Log<jats:italic>X<\/jats:italic>, <jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>\u00a0=\u00a00.9933), and the average recovery is among 91.00% to 106.39% without cross\u2010reactions with the other canine viral antigen. The correlation between ELISA assay and this method is up to 0.9861. And, there is high correlation between the CPV\u20102\u2010Ag concentration and antibody titers (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>\u00a0=\u00a00.9234). This immunoassay established has high sensitivity, accuracy, and specificity, which indicate that this method could be suitable for quantitative detection of CPV\u20102\u2010Ag in vaccine evaluation.<\/jats:p>","DOI":"10.1002\/bab.1967","type":"journal-article","created":{"date-parts":[[2020,6,13]],"date-time":"2020-06-13T13:13:20Z","timestamp":1592054000000},"page":"597-602","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Determination of parvovirus antigen in the vaccine using time\u2010resolved fluorescence immunoassay"],"prefix":"10.1002","volume":"68","author":[{"given":"Cuicui","family":"Chen","sequence":"first","affiliation":[{"name":"Guangzhou Youdi Bio\u2010Technology Co., Ltd.  Guangzhou 510663 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huankun","family":"Liang","sequence":"additional","affiliation":[{"name":"Guangzhou Youdi Bio\u2010Technology Co., Ltd.  Guangzhou 510663 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baoyu","family":"Hu","sequence":"additional","affiliation":[{"name":"Guangzhou Youdi Bio\u2010Technology Co., Ltd.  Guangzhou 510663 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Ning","sequence":"additional","affiliation":[{"name":"Guangzhou Youdi Bio\u2010Technology Co., Ltd.  Guangzhou 510663 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongrui","family":"Lai","sequence":"additional","affiliation":[{"name":"Guangzhou Youdi Bio\u2010Technology Co., Ltd.  Guangzhou 510663 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"He","sequence":"additional","affiliation":[{"name":"Guangzhou Youdi Bio\u2010Technology Co., Ltd.  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