{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T05:59:49Z","timestamp":1788328789877,"version":"build-2803163510"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,8,16]],"date-time":"2020-08-16T00:00:00Z","timestamp":1597536000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An increasing number of studies have associated nuclear factor\u2010\u03baB (NF\u2010\u03baB) activation to the progression of myocardial infarction (MI). Multiple members of the ubiquitin\u2010specific protease (USP) family regulate the NF\u2010\u03baB signaling pathway. This study attempted to investigate the function and underlying mechanism of USP family members in MI. Monocytes were isolated from peripheral blood samples of MI and healthy control, and the expression of several USP family members and NF\u2010\u03baB was examined. After USP47 knockdown or overexpression, cell apoptosis, related protein levels, and NF\u2010\u03baB promoter activity were measured. We found that USP47 and NF\u2010\u03baB expression was significantly increased in MI, and USP47 was positively correlated with NF\u2010\u03baB. In a rat H9c2 myocardial cell ischemia\/reperfusion (I\/R) injury model, USP47 expression was increased in a time\u2010dependent manner. USP47 knockdown decreased I\/R injury\u2010induced apoptosis, with increased survivin and cytoplasmic NF\u2010\u03baB and decreased cleaved caspase\u20103 and nuclear NF\u2010\u03baB. USP47\u2010induced apoptosis was potently counteracted by the NF\u2010\u03baB inhibitor, and cytoplasmic NF\u2010\u03baB was increased and nuclear NF\u2010\u03baB was decreased. Luciferase reporter showed that USP47 promotes NF\u2010\u03baB promoter activity. These results suggested that USP47 expression may be correlated with MI progression. USP47 knockdown attenuated myocardial cell I\/R injury by inhibiting apoptosis.<\/jats:p>","DOI":"10.1002\/bab.2000","type":"journal-article","created":{"date-parts":[[2020,8,6]],"date-time":"2020-08-06T09:34:10Z","timestamp":1596706450000},"page":"841-848","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["USP47 promotes apoptosis in rat myocardial cells after ischemia\/reperfusion injury via NF\u2010\u03baB activation"],"prefix":"10.1002","volume":"68","author":[{"given":"Yu","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Medicine Shanghai Sixth People's Hospital East Affiliated to Shanghai University of Medicine &amp; Health Sciences  Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihui","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine Shanghai Sixth People's Hospital 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