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In this study, the anticancer properties of <jats:italic>G. neo\u2010japonicum<\/jats:italic> were evaluated using cellular and computational models. The ethanolic extract (EtOH) with a promising inhibitory effect was fractionated into four different fractions: hexane (Hex), chloroform (Chl), butanol (Btn), and aqueous (Aq). The active fractions were then subjected to cell apoptosis assessment and phytochemical profiling. Molecular docking was conducted to elucidate the affinity of selected constituents towards antiapoptotic Bcl\u20102 protein. The butanol fraction showed the highest antioxidant activities as well as total phenolic content. Both hexane and chloroform fractions exerted a potent cytotoxic effect on colonic carcinoma cells through the induction of apoptosis. Phytochemical analysis revealed that the chloroform fraction is terpenoid enriched whereas the hexane fraction comprises predominantly sterol constituents. Stellasterol and 1,25\u2010dihydroxyvitamin D3 3\u2010glycoside were demonstrated to have a high affinity towards Bcl\u20102 protein. Overall, <jats:italic>G. neo\u2010japonicum<\/jats:italic> can be considered as a compelling therapeutic candidate for cancer treatment.<\/jats:p>","DOI":"10.1002\/bab.2013","type":"journal-article","created":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T18:25:43Z","timestamp":1598639143000},"page":"902-917","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["<i>In vitro<\/i> and <i>in silico<\/i> anticancer evaluation of a medicinal mushroom, <i>Ganoderma neo\u2010japonicum<\/i> Imazeki, against human colonic carcinoma cells"],"prefix":"10.1002","volume":"68","author":[{"given":"Meng\u2010Fei","family":"Lau","sequence":"first","affiliation":[{"name":"Department of Biomedical Science Faculty of Medicine University of Malaya  Kuala Lumpur 50603 Malaysia"},{"name":"Mushroom Research Centre University of Malaya  Kuala Lumpur 50603 Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kek\u2010Heng","family":"Chua","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science Faculty of Medicine University of Malaya  Kuala Lumpur 50603 Malaysia"},{"name":"Mushroom Research Centre University of Malaya  Kuala Lumpur 50603 Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vikineswary","family":"Sabaratnam","sequence":"additional","affiliation":[{"name":"Mushroom Research Centre University of Malaya  Kuala Lumpur 50603 Malaysia"},{"name":"Institute of Biological Science Faculty of Science University of Malaya  Kuala Lumpur 50603 Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8900-7476","authenticated-orcid":false,"given":"Umah Rani","family":"Kuppusamy","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science Faculty of Medicine University of Malaya  Kuala Lumpur 50603 Malaysia"},{"name":"Mushroom Research Centre University of Malaya  Kuala Lumpur 50603 Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,9,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.01217"},{"key":"e_1_2_8_3_1","first-page":"283","volume":"13","author":"Kardooni R.","year":"2014","journal-title":"Indian J. 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