{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,12]],"date-time":"2025-02-12T19:10:19Z","timestamp":1739387419228,"version":"3.37.0"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,12,8]],"date-time":"2020-12-08T00:00:00Z","timestamp":1607385600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003093","name":"Ministry of Higher Education, Malaysia","doi-asserted-by":"publisher","award":["HICoE: 311\/CIPPM\/4401005"],"award-info":[{"award-number":["HICoE: 311\/CIPPM\/4401005"]}],"id":[{"id":"10.13039\/501100003093","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Lymphatic filariasis is a neglected parasitic disease that affects millions in tropical and subtropical countries and is caused by <jats:italic>Wuchereria<\/jats:italic> and <jats:italic>Brugia<\/jats:italic> species. Specific and sensitive detection methods are essential in mapping infected areas where rapid tests are needed to cover underdeveloped and remote regions, which facilitates eliminating the disease as a public health problem. A few commercialized rapid tests based on antigen or antibody detection are available, but the former only detects infection by <jats:italic>Wuchereria<\/jats:italic> species and cross\u2010reacts with nonlymphatic filaria, whereas antibody detection might provide positive results of previous infection. Here, we report the production of three different recombinant immunoglobulin gamma (IgG)1 antibodies based on scFvs previously generated via human antibody phage display technology, that is, anti\u2010BmR1 clone 4, anti\u2010BmXSP clone 5B, and anti\u2010BmXSP clone 2H2. The scFv sequences were cloned into a pCMV\u2010IgG1 vector, then transfected into a HEK293F cell line. The generated antibodies were found to be able to bind to their respective targets even at relatively low concentration. Conjugation of Fc to scFv induces binder stability and provides multiple labeling sites for probes and signaling molecules that can be used in rapid tests.<\/jats:p>","DOI":"10.1002\/bab.2082","type":"journal-article","created":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T08:23:02Z","timestamp":1606810982000},"page":"70-76","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Generation of human scFv\u2013IgG1Fc antibodies for detection of lymphatic filarial recombinant antigens, BmR1 and BmSXP"],"prefix":"10.1002","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6208-3791","authenticated-orcid":false,"given":"Ahmad Ismail Khaled","family":"Abdo","sequence":"first","affiliation":[{"name":"Institute for Research in Molecular Medicine (INFORMM) Universiti Sains Malaysia  Minden Penang Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying Yuan","family":"Ngoh","sequence":"additional","affiliation":[{"name":"Institute for Research in Molecular Medicine (INFORMM) Universiti Sains Malaysia  Minden Penang Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min Han","family":"Lew","sequence":"additional","affiliation":[{"name":"Institute for Research in Molecular Medicine (INFORMM) Universiti Sains Malaysia  Minden Penang Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvia Annabel","family":"Dass","sequence":"additional","affiliation":[{"name":"Institute for Research in Molecular Medicine (INFORMM) Universiti Sains Malaysia  Minden Penang Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anizah","family":"Rahumatullah","sequence":"additional","affiliation":[{"name":"Institute for Research in Molecular Medicine (INFORMM) Universiti Sains Malaysia  Minden Penang Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rahmah","family":"Noordin","sequence":"additional","affiliation":[{"name":"Institute for Research in Molecular Medicine (INFORMM) Universiti Sains Malaysia  Minden Penang Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6707-9505","authenticated-orcid":false,"given":"Gee Jun","family":"Tye","sequence":"additional","affiliation":[{"name":"Institute for Research in Molecular Medicine (INFORMM) Universiti Sains Malaysia  Minden Penang Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,12,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1055\/a-0918-3678"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-1-4160-4390-4.00110-7"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006963"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2008.02.007"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000326"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(99)00231-5"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2883-3-10"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2015.07.040"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18112376"},{"issue":"4","key":"e_1_2_10_11_1","first-page":"256","volume":"50","author":"Khoo T. 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