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To remove the excess sorbitol from the body, proposed binding of sorbitol with caffeine was investigated. Their interaction in serum medium was studied and established by UV\u2010Vis, fluorescence spectrophotometry, and time\u2010correlated single photon counting (TCSPC). The linear calibration of sorbitol (in the range 10\u201350 mM) was done using UV\u2010Vis spectrophotometry. Time scan experiments furnished the reaction rate of sorbitol assayed solution as well as sorbitol\u2013caffeine complex as 0.021 min<jats:sup>\u22121<\/jats:sup> and 0.018 min<jats:sup>\u22121<\/jats:sup>, respectively. A sudden drop was observed in the fluorescence lifetime of reduced nicotinamide adenine dinucleotide (NADH) present in sorbitol assayed solution upon complexation with caffeine, that is, from 1.774 \u00d7 10<jats:sup>\u221209<\/jats:sup> to 1.23 \u00d7 10<jats:sup>\u221210<\/jats:sup> Sec, which indicates the hindrance in the formation of NADH and the probable formation of some other species. Isothermal titration calorimetric experiments clearly indicate the number of binding sites (i.e., 3.89, 1.40, and 2.07) that exist between sorbitol and caffeine at the complexation ratio of 1:1.2, 1:1.5, and 1:3. The present method can be helpful in pharmacological and therapeutic studies of sorbitol using caffeine for treating diabetic neuropathy.<\/jats:p>","DOI":"10.1002\/bab.2083","type":"journal-article","created":{"date-parts":[[2020,12,2]],"date-time":"2020-12-02T22:49:21Z","timestamp":1606949361000},"page":"77-91","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Free serum sorbitol and its interaction with caffeine: A suggestive approach for plausible remediation of diabetic neuropathy"],"prefix":"10.1002","volume":"69","author":[{"given":"Bhavya","family":"Srivastava","sequence":"first","affiliation":[{"name":"Department of Chemistry University of Calcutta  92, APC Road Kolkata West Bengal 700009 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Souvik","family":"Sen","sequence":"additional","affiliation":[{"name":"KPC Medical College &amp; Hospital  1F, Raja Subodh Chandra Mullick Road, Jadavpur Kolkata West Bengal 700032 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1005-5147","authenticated-orcid":false,"given":"Kamalika","family":"Sen","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Calcutta  92, APC Road Kolkata West Bengal 700009 India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,12,28]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-37186-2"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/5825971"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.4239\/wjd.v7.i17.342"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc16-2042"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.2174\/1570161116666180829120101"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.02.105"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2019.06.057"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-815876-0.00019-X"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1042\/BCJ20160503C"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.2337\/db19-574-P"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdi.13190"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1139\/apnm-2016-0433"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/ame2.12001"},{"key":"e_1_2_11_15_1","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/B978-0-12-803620-4.00017-7","volume-title":"Biomarkers in Nonclinical Drug Development, A Comprehensive Guide to Toxicology in Nonclinical Drug Development","author":"Aulbach A. 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