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Because of its toxicity, the cyanide\u2010containing effluents from industries have caused many environmental problems. Among various methods to treat the contaminated soils or water, the biological degradation is regarded to be promising. We isolated two cyanide\u2010degrading microorganisms, <jats:italic>Pedobacter<\/jats:italic> sp. EBE\u20101 and <jats:italic>Bacillus<\/jats:italic> sp. EBE\u20102, from soil contaminated with cyanide. Among these bacteria, <jats:italic>Bacillus<\/jats:italic> sp. EBE\u20102 exhibited significantly a high cyanide\u2010degrading ability. <jats:italic>Bacillus<\/jats:italic> sp. EBE\u20102 might be used for the remediation of cyanide contaminated water or soil. A nitrilase gene was cloned from <jats:italic>Bacillus<\/jats:italic> sp. EBE\u20102. <jats:italic>Bacillus<\/jats:italic> nitrilase was expressed in <jats:italic>Escherichia coli<\/jats:italic> and purified. <jats:italic>Bacillus<\/jats:italic> nitrilase exhibited cyanide\u2010degrading activity as a large oligomer. Since formic acid formation from cyanide was observed, <jats:italic>Bacillus<\/jats:italic> nitrilase is likely to be a cyanide hydrolase. Although there exist various homologous enzymes annotated as carbon\u2013nitrogen family hydrolases, this is the first report on the cyanide degrading activity. The structure and catalytic site of <jats:italic>Bacillus<\/jats:italic> nitrilase were studied by homology modeling and molecular docking simulation.<\/jats:p>","DOI":"10.1002\/bab.2095","type":"journal-article","created":{"date-parts":[[2020,12,30]],"date-time":"2020-12-30T14:38:43Z","timestamp":1609339123000},"page":"183-189","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Isolation of cyanide\u2010degrading bacteria and molecular characterization of its cyanide\u2010degrading nitrilase"],"prefix":"10.1002","volume":"69","author":[{"given":"Ayane","family":"Terada","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Life Science Tokyo University of Agriculture and Technology  Koganei Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisuke","family":"Komatsu","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Life Science Tokyo University of Agriculture and Technology  Koganei Tokyo Japan"},{"name":"EnBio Engineering  Chiyoda Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takahiro","family":"Ogawa","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Life Science Tokyo University of Agriculture and Technology  Koganei Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darin","family":"Flamandita","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Universitas Indonesia  Depok Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6360-3691","authenticated-orcid":false,"given":"Muhamad","family":"Sahlan","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Universitas Indonesia  Depok Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minoru","family":"Nishimura","sequence":"additional","affiliation":[{"name":"EnBio Engineering  Chiyoda Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8307-9671","authenticated-orcid":false,"given":"Masafumi","family":"Yohda","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Life Science Tokyo University of Agriculture and Technology  Koganei Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,1,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pa.24.040184.002315"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","unstructured":"M. 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