{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T11:37:50Z","timestamp":1761824270036,"version":"3.37.0"},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,1,24]],"date-time":"2021-01-24T00:00:00Z","timestamp":1611446400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Angiotensin\u2010converting enzyme (ACE) liable for the regulation of blood pressure was purified from human plasma by affinity chromatography. Impact of water and butanol extracts of <jats:italic>Matricaria chamomilla<\/jats:italic> L. on purity ACE was examined. ACE was purified using the affinity chromatography method. The enzyme activity was evaluated at 345\u00a0nm by a <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/spectrophotometer\">spectrophotometer<\/jats:ext-link>. Extracts of <jats:italic>M. chamomilla<\/jats:italic> plant with butanol and water were prepared. Lisinopril was utilized as a specific inhibitor. ACE was purified 3,659\u2010fold from human plasma and the specific activity was 1,350\u00a0EU\/mg protein. The molecular weight and purity of ACE were found by SDS\u2010PAGE and two bands of 60 and 70\u00a0kDa on the gel were detected. Water and butanol extracts of <jats:italic>M. chamomilla<\/jats:italic> demonstrated inhibitor impact on ACE activity. IC<jats:sub>50<\/jats:sub> constants for water and butanol extracts of <jats:italic>M. chamomilla<\/jats:italic> were computed to be 1.292 and 0.353\u00a0mg\/mL, respectively. The type of inhibition for whole inhibitors was identified as noncompetitive. IC<jats:sub>50<\/jats:sub> and <jats:italic>K<\/jats:italic><jats:sub>i<\/jats:sub> constants for lisinopril were calculated to be 0.781 and 0.662\u00a0nM, respectively. These results indicate that butanol and water extracts of <jats:italic>M. chamomilla<\/jats:italic> may have an ACE inhibitor potential.<\/jats:p>","DOI":"10.1002\/bab.2106","type":"journal-article","created":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T12:55:38Z","timestamp":1610715338000},"page":"273-280","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Investigation of inhibition effect of butanol and water extracts of <i>Matricaria chamomilla<\/i> L. on angiotensin\u2010converting enzyme purified from human plasma"],"prefix":"10.1002","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4071-9744","authenticated-orcid":false,"given":"Zehra","family":"Bas","sequence":"first","affiliation":[{"name":"Department of Nutrition and Dietetics, Faculty of Health Sciences Van Y\u00fcz\u00fcnc\u00fc Y\u0131l University  Van Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0935-1195","authenticated-orcid":false,"given":"Vedat","family":"Turkoglu","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science Van Y\u00fcz\u00fcnc\u00fcY\u0131l University  Van Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1040-9964","authenticated-orcid":false,"given":"Yasar","family":"Goz","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Dietetics, Faculty of Health Sciences Van Y\u00fcz\u00fcnc\u00fc Y\u0131l University  Van Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,1,24]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_7_2_1","DOI":"10.3390\/nu9040316"},{"doi-asserted-by":"publisher","key":"e_1_2_7_3_1","DOI":"10.1016\/S0378-8741(02)00037-5"},{"doi-asserted-by":"publisher","key":"e_1_2_7_4_1","DOI":"10.1021\/bi00782a007"},{"doi-asserted-by":"publisher","key":"e_1_2_7_5_1","DOI":"10.1016\/S0002-9343(03)00090-1"},{"doi-asserted-by":"publisher","key":"e_1_2_7_6_1","DOI":"10.1016\/j.jep.2008.06.003"},{"doi-asserted-by":"publisher","key":"e_1_2_7_7_1","DOI":"10.1016\/S0378-8741(98)00201-3"},{"doi-asserted-by":"publisher","key":"e_1_2_7_8_1","DOI":"10.1016\/j.foodchem.2014.05.080"},{"doi-asserted-by":"publisher","key":"e_1_2_7_9_1","DOI":"10.1016\/j.ejmech.2019.06.091"},{"doi-asserted-by":"publisher","key":"e_1_2_7_10_1","DOI":"10.1016\/j.phymed.2017.10.008"},{"doi-asserted-by":"publisher","key":"e_1_2_7_11_1","DOI":"10.1111\/bph.14333"},{"doi-asserted-by":"publisher","key":"e_1_2_7_12_1","DOI":"10.1021\/acs.jafc.8b00099"},{"doi-asserted-by":"publisher","key":"e_1_2_7_13_1","DOI":"10.1016\/j.phymed.2018.03.034"},{"doi-asserted-by":"publisher","key":"e_1_2_7_14_1","DOI":"10.1016\/j.cbi.2017.05.025"},{"key":"e_1_2_7_15_1","first-page":"44","volume-title":"Herbal Medicine and Botanical Medical Fads","author":"Hoffmann F. 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