{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T21:01:54Z","timestamp":1774472514810,"version":"3.50.1"},"reference-count":82,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,1,28]],"date-time":"2021-01-28T00:00:00Z","timestamp":1611792000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Oxidative stress is to upregulate the pentose phosphate pathway (PPP). The PPP consists of two functional branches, glucose\u20106\u2010phosphate dehydrogenase (G6PD) and 6\u2010phosphogluconaste dehydrogenase (6PGD). Glutathione reductase (GR) has a significant role in catalyzing an oxidized glutathione form into a reduced form. The purpose of this study is to investigate the effects of brimonidine and proparacaine on the activity of 6PGD, G6PD, and GR enzymes purified from human erythrocytes. Brimonidine displayed considerable inhibition profile against G6PD with IC<jats:sub>50<\/jats:sub> value and <jats:italic>K<\/jats:italic><jats:sub>I<\/jats:sub> constant of 29.93\u00a0\u00b1\u00a03.56 and 48.46\u00a0\u00b1\u00a00.66\u00a0\u03bcM, respectively. On the other hand, proparacaine had no inhibitory effect against G6PD. <jats:italic>K<\/jats:italic><jats:sub>I<\/jats:sub> values were found to be 66.06\u00a0\u00b1\u00a00.78 and 811.50\u00a0\u00b1\u00a011.13\u00a0\u03bcM for brimonidine and proparacaine, respectively, for 6PGD. <jats:italic>K<\/jats:italic><jats:sub>I<\/jats:sub> values were found to be 144.10\u00a0\u00b1\u00a02.01 and 1,654.00\u00a0\u00b1\u00a026.29\u00a0\u03bcM for brimonidine and proparacaine, respectively, for GR. Herein, also <jats:italic>in silico<\/jats:italic> molecular docking studies were performed between drugs and enzymes.<\/jats:p>","DOI":"10.1002\/bab.2107","type":"journal-article","created":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T13:07:37Z","timestamp":1610716057000},"page":"281-288","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["The effect of brimonidine and proparacaine on metabolic enzymes: Glucose\u20106\u2010phosphate dehydrogenase, 6\u2010phosphogluconate dehydrogenase, and glutathione reductase"],"prefix":"10.1002","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2350-184X","authenticated-orcid":false,"given":"B\u00fc\u015fra","family":"\u00c7al\u0131\u015fkan","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Faculty of Medicine Atat\u00fcrk University  Erzurum Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2603-7509","authenticated-orcid":false,"given":"Arzu","family":"\u00d6zt\u00fcrk Kesebir","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science Atat\u00fcrk University  Erzurum Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3216-1098","authenticated-orcid":false,"given":"Yeliz","family":"Demir","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Services, Nihat Delibalta G\u00f6le Vocational High School Ardahan University  Ardahan Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2079-6213","authenticated-orcid":false,"given":"\u0130lknur","family":"Akyol Salman","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Faculty of Medicine Atat\u00fcrk University  Erzurum Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,1,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929866526666190226162225"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2019.09.237"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12010-019-03009-x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.17-23402"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1011-1344(01)00196-8"},{"issue":"2","key":"e_1_2_6_7_1","first-page":"9","volume":"61","author":"Tenkate T. 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