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HFF, an important reproductive fluid, was used for the proteomic analysis of PCOS patients to determine the effect of PCOS on folliculogenesis. HFF was obtained from 10 women (average age, 35 years) undergoing in vitro fertilization at two hospitals. The proteins of HFF were separated using 2\u2010DE analysis and validated by Western blot assay. Approximately 250 protein spots were separated on the 2\u2010DE gel. Among them, the expression levels of seven proteins were found to change at least 1.5\u2010fold in the PCOS patient group. Three protein spots, albumin, uncharacterized protein 1, and uncharacterized protein 2, were downregulated in PCOS patients. However, four protein spots, gelsolin, vitamin D binding protein, serum albumin, and complement factor B, were upregulated in PCOS patient group. These proteins may serve as a panel of potential pathological biomarkers during fertilization and oocyte development.<\/jats:p>","DOI":"10.1002\/bab.2108","type":"journal-article","created":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T11:59:00Z","timestamp":1610711940000},"page":"289-295","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Proteomic analysis of human follicular fluid from polycystic ovary syndrome patients"],"prefix":"10.1002","volume":"69","author":[{"given":"Young\u2010Jin","family":"Sim","sequence":"first","affiliation":[{"name":"Elle Medi Obstetrics  Changwon Gyeongnam 51191 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0330-8620","authenticated-orcid":false,"given":"A\u2010Reum","family":"Ryu","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology Soonchunhyang University  Asan 31538 Republic of Korea"},{"name":"Department of Medical Science Soonchunhyang University  Asan 31538 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3511-9522","authenticated-orcid":false,"given":"Mi\u2010Young","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology Soonchunhyang University  Asan 31538 Republic of Korea"},{"name":"Department of Medical Science Soonchunhyang University  Asan 31538 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,1,20]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/del257"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.109.082941"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.5935\/1518-0557.20160009"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/aogs.13805"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcna.2008.04.008"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecl.2013.10.003"},{"key":"e_1_2_9_8_1","first-page":"1","volume":"13","author":"Bannigida D. 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