{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T09:03:43Z","timestamp":1769245423962,"version":"3.49.0"},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A previous study has elucidated that circular RNA circCLK3 acts as an oncogenic gene in cervical cancer. However, the role and regulatory mechanism of circCLK3 in tongue squamous cell carcinoma (TSCC) remain unknown. Quantitative real\u2010time PCR was used to examine targeted gene expression in different groups. Cell viability and proliferation were investigated by MTT and 5\u2010ethynyl\u20102\u2032\u2010deoxyuridine assays. Cell migration and invasion were detected by Transwell assays, and cell apoptosis was measured by flow cytometry analysis. The interaction among genes was investigated using luciferase reporter assay, RNA pull\u2010down assay, and RNA immunoprecipitation assay. In the present study, our findings revealed the upregulated expression of circCLK3 in TSCC tissues and cell lines. CircCLK3 knockdown suppressed cell proliferation, migration invasion, and induced cell cycle arrest at G0\/G1 phase in TSCC. Moreover, circCLK3 acted as a molecular sponge for miR\u2010455\u20105p. PARVA was the target gene of miR\u2010455\u20105p. Furthermore, the negative correlation between expression of miR\u2010455\u20105p and circCLK3 or PARVA in TSCC tissues was discovered. Rescue assays indicated that PARVA overexpression reversed the circCLK3 knockdown\u2010mediated inhibitory effects on the progression of TSCC. In summary, circCLK3 exerts its carcinogenic effects on TSCC progression via absorbing miR\u2010455\u20105p to upregulate PARVA, which expands our knowledge on the underlying mechanism of TSCC.<\/jats:p>","DOI":"10.1002\/bab.2120","type":"journal-article","created":{"date-parts":[[2021,3,3]],"date-time":"2021-03-03T09:16:37Z","timestamp":1614762997000},"page":"431-441","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Circular RNA circCLK3 promotes the progression of tongue squamous cell carcinoma via miR\u2010455\u20105p\/PARVA axis"],"prefix":"10.1002","volume":"69","author":[{"given":"Huiming","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Stomatology The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University  Huaian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhifen","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Stomatology Huaian Maternal and Child Health Hospital  Huaian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaowei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Oncology The Affiliated Huaian No.1 People\u2019s Hospital of Nanjing Medical University  Huaian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5073-5329","authenticated-orcid":false,"given":"Dazhao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Stomatology The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University  Huaian China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,4]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.14189"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/00016489.2017.1279751"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.28869"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oooo.2019.08.011"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-019-5859-y"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2015.04.024"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envres.2019.108825"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10741-019-09908-9"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-019-1483-6"},{"key":"e_1_2_11_11_1","first-page":"e999","article-title":"Circular rna circ\u2010cmpk1 contributes to cell proliferation of non\u2010small cell lung cancer by elevating cyclin d1 via sponging mir\u2010302e","author":"Cui D.","year":"2019","journal-title":"Mol Genet Genomic Med."},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.102519"},{"key":"e_1_2_11_13_1","first-page":"10300","article-title":"Circ_0001742 promotes tongue squamous cell carcinoma progression via mir\u2010431\u20105p\/atf3 axis","volume":"23","author":"Hu Y. 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