{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:54:06Z","timestamp":1789606446722,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,3,26]],"date-time":"2021-03-26T00:00:00Z","timestamp":1616716800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Osteoarthritis (OA) is a common joint disease. Currently, many studies have revealed that circular RNAs (circRNAs) are strongly related to the occurrence and development of diseases. Hence, we aimed to further elucidate the role and molecular mechanism of circRNA SEC24 homolog A, COPII coat complex component (circSEC24A) in OA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Chondrocytes were treated with interleukin\u20101\u03b2 (IL\u20101\u03b2) to establish OA cell model in vitro. The expression levels of circSEC24A, microRNA\u2010142\u20105p (miR\u2010142\u20105p), and sex\u2010determining region Y\u2010box protein 5 (SOX5) were determined by quantitative real\u2010time polymerase chain reaction. MTT and colony formation assays were used to determine cell proliferation. Cell apoptosis was detected by flow cytometry analysis. The protein levels of inflammatory factors and SOX5 were determined by western blot assay. The relationship between miR\u2010142\u20105p and circSEC24A or SOX5 was confirmed using dual\u2010luciferase reporter assay and RNA immunoprecipitation assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CircSEC24A and SOX5 expression were enhanced, while miR\u2010142\u20105p level was reduced in OA cartilage tissues and chondrocytes. Overexpression of circSEC24A promoted IL\u20101\u03b2\u2010induced injury through decreasing cell proliferation and increasing apoptosis and inflammation in chondrocytes. MiR\u2010142\u20105p was a direct target of circSEC24A, and its upregulation ameliorated IL\u20101\u03b2\u2010induced injury and abated the effect of oe\u2010circSEC24A in IL\u20101\u03b2\u2010induced chondrocytes. Additionally, SOX5 was a downstream target of miR\u2010142\u20105p, and its overexpression had a similar role with oe\u2010circSEC24A and reversed the impact of miR\u2010142\u20105p in IL\u20101\u03b2\u2010induced chondrocytes. CircSEC24A acted as a molecular sponge of miR\u2010142\u20105p to regulate SOX5 expression in chondrocytes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>CircSEC24A aggravated IL\u20101\u03b2\u2010induced injury via modulating miR\u2010142\u20105p\/SOX5 axis, providing possible targets for the clinical diagnosis and treatment of OA.<\/jats:p><\/jats:sec>","DOI":"10.1002\/bab.2145","type":"journal-article","created":{"date-parts":[[2021,3,10]],"date-time":"2021-03-10T07:28:30Z","timestamp":1615361310000},"page":"701-713","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["CircSEC24A promotes IL\u20101\u03b2\u2010induced apoptosis and inflammation in chondrocytes by regulating miR\u2010142\u20105p\/SOX5 axis"],"prefix":"10.1002","volume":"69","author":[{"given":"Lin","family":"Shi","sequence":"first","affiliation":[{"name":"Department of Hand and Foot Orthopedic Surgery Weifang People's 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