{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T04:15:00Z","timestamp":1788149700704,"version":"build-2803163510"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T00:00:00Z","timestamp":1620086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T00:00:00Z","timestamp":1620086400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The Bacillus Calmette\u2010Gu\u00e9rin (BCG) vaccine has been used to prevent tuberculosis (TB), but it cannot prevent adults against TB. The\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    Beijing strain is the most popular strain in China, but no vaccine is designed for the Beijing strain. It is vital to design a multiepitopes\u2010based vaccine against the Beijing strain for the Chinese population. The bioinformatics tools were used to predict CD4+ T\u2010cell epitopes in five protective antigens based on the Chinese population\u2010specific alleles. The antigenicity, allergenicity, toxicity, IFN\u2010\u03b3 level, population coverage, and three\u2010dimensional structure were predicted using Vaxijen, AllerTOP, ToxinPred, IFN\u2010\u03b3 epitope server, IEDB, and I\u2010TASSER, respectively. One\u2010hundred one promiscuous epitopes were obtained from Rv1813c, Rv2608, Rv3131, and Rv3628 proteins. After screening with antigenicity, allergenicity, toxicity, and IFN\u2010\u03b3 level, seven epitopes from Rv2608 and Rv3131 proteins were selected to be vaccine candidates. Further study determined their three\u2010dimensional structure and the coverage in the Chinese population as high as 99%. Our study predicted seven CD4+ T\u2010cell dominant epitopes from the proteins Rv2608 and Rv3131 of\n                    <jats:italic>M. tuberculosis<\/jats:italic>\n                    Beijing strain for the first time, which may provide a basis for improving the design of multiepitopes\u2010based vaccines for TB.\n                  <\/jats:p>","DOI":"10.1002\/bab.2171","type":"journal-article","created":{"date-parts":[[2021,4,22]],"date-time":"2021-04-22T17:09:33Z","timestamp":1619111373000},"page":"1002-1014","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Prediction and analyses of HLA\u2010II restricted\n                    <i>Mycobacterium tuberculosis<\/i>\n                    CD4\n                    <sup>+<\/sup>\n                    T cell epitopes in the Chinese 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