{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T02:53:21Z","timestamp":1791860001829,"version":"4.3.4"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Streptomyces nodosus<\/jats:italic>\n                    is known as the main manufacturer of amphotericin B (AmB), which is an effective antifungal drug. It is verified that the optimization of fermentation conditions and key growth factors have a great impact on the yield of AmB. The AmB production of\n                    <jats:italic>S. nodosus<\/jats:italic>\n                    in cotton\u2010seed meal (CM) medium was 1.6 times than that of beef\u2010paste medium. The transcriptome analysis was used to analyze the effects of different nitrogen media and calcium on\n                    <jats:italic>S. nodosus<\/jats:italic>\n                    . Related genes of the EMP and TCA pathways, such as phosphofructokinase, pyruvate dehydrogenase, and citrate synthase, were upregulated in CM medium. The expression level of the PKS modules of the amphotericin synthesis gene cluster in beef\u2010paste medium was higher. Other functional genes, such as amphGH and amphRIV, have the advantage of expressing in CM medium. Ca\n                    <jats:sup>2+<\/jats:sup>\n                    promoted the upregulation of genes in metabolic pathways such as EMP pathway (pyruvate dehydrogenase), TCA pathway (citrate synthase), and amphotericin synthesis genes (PKS modules). The expression of WhiB family genes\n                    <jats:italic>SNOD<\/jats:italic>\n                    _RS 13310 and\n                    <jats:italic>SNOD<\/jats:italic>\n                    _RS 17625 was positively correlated with Ca\n                    <jats:sup>2+<\/jats:sup>\n                    concentration. In addition, in the presence of calcium, the expression level of Sec transport system genes of\n                    <jats:italic>S. nodosus<\/jats:italic>\n                    was lower.\n                  <\/jats:p>","DOI":"10.1002\/bab.2221","type":"journal-article","created":{"date-parts":[[2021,7,12]],"date-time":"2021-07-12T17:28:45Z","timestamp":1626110925000},"page":"1489-1501","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Analysis of the effects of different nitrogen sources and calcium on the production of amphotericin by\n                    <i>Streptomyces nodosus<\/i>\n                    based on comparative transcriptome"],"prefix":"10.1002","volume":"69","author":[{"given":"Kai","family":"Huang","sequence":"first","affiliation":[{"name":"The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals Zhejiang University of Technology  Hangzhou China"},{"name":"Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering Zhejiang University of Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Zhang","sequence":"additional","affiliation":[{"name":"The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals Zhejiang University of Technology  Hangzhou China"},{"name":"Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering Zhejiang University of Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Chen","sequence":"additional","affiliation":[{"name":"The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals Zhejiang University of Technology  Hangzhou China"},{"name":"Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering Zhejiang University of Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe\u2010Ming","family":"Wu","sequence":"additional","affiliation":[{"name":"The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals Zhejiang University of Technology  Hangzhou China"},{"name":"Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering Zhejiang University of Technology  Hangzhou China"},{"name":"Zhejiang Tiantai Pharmaceutical Co., Ltd. Taizhou  Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3259-6796","authenticated-orcid":false,"given":"Zhi\u2010Qiang","family":"Liu","sequence":"additional","affiliation":[{"name":"The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals Zhejiang University of Technology  Hangzhou China"},{"name":"Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering Zhejiang University of Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6358-6243","authenticated-orcid":false,"given":"Yu\u2010Guo","family":"Zheng","sequence":"additional","affiliation":[{"name":"The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals Zhejiang University of Technology  Hangzhou China"},{"name":"Key Laboratory of Bioorganic Synthesis of Zhejiang 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