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CTX\u2010B is a relatively small molecule (12\u00a0kDa) and it was chosen as model analyte for the detection of protein toxins originated from waterborne pathogens. Recognition layer was prepared on gold\u2010coated LaSFN9 glasses modified with 11\u2010mercaptoundecanoic acid (11\u2010MUA). Biotin\u2010conjugated anti\u2010CTX\u2010B polyclonal antibody (B\u2010Ab) was immobilized on streptavidin (SA) layer constructed on the 11\u2010MUA\u2010modified surface. CTX\u2010B amount was determined with direct assay using B\u2010Ab in surface plasmon resonance (SPR) mode and with sandwich assay in SPFS mode using Cy5\u2010conjugated anti\u2010CTX\u2010B polyclonal antibody. Minimum detected CTX\u2010B concentrations were 10 and 0.01\u00a0\u03bcg\/ml with SPR and SPFS, respectively, showing the sensitivity of the SPFS system over the conventional one. The detection was done in 2\u20136\u00a0h, which was faster than both culture and polymerase chain reaction (PCR)\u2010based methods. Stability tests were performed with SA\u2010coated sensors (excluding B\u2010Ab). In this form, the layer was stable after 30\u00a0days of storage in phosphate\u2010buffered saline (PBS; 0.01\u00a0M, pH = 7.4) at +4\u00b0C. B\u2010Ab layer was formed immediately on them before each measurement.<\/jats:p>","DOI":"10.1002\/bab.2227","type":"journal-article","created":{"date-parts":[[2021,7,23]],"date-time":"2021-07-23T09:07:47Z","timestamp":1627031267000},"page":"1557-1566","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Detection of cholera toxin with surface plasmon field\u2010enhanced fluorescent spectroscopy"],"prefix":"10.1002","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3554-4134","authenticated-orcid":false,"given":"Sebnem","family":"Seherler","sequence":"first","affiliation":[{"name":"Molecular Biology\u2010Genetics and Biotechnology Programme Istanbul Technical University  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anil","family":"Bozdogan","sequence":"additional","affiliation":[{"name":"Department of Material Science and Engineering Gebze Technical University  Kocaeli Turkey"},{"name":"Center for Electrochemical Surface Technology (CEST) Austrian Institute of Technology  Tulln Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tugba Arzu","family":"Ozal Ildeniz","sequence":"additional","affiliation":[{"name":"Department of Medical Engineering, Faculty of Engineering Acibadem Mehmet Ali Aydinlar University  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5093-6013","authenticated-orcid":false,"given":"Fatma Nese","family":"Kok","sequence":"additional","affiliation":[{"name":"Molecular Biology\u2010Genetics and Biotechnology Programme Istanbul Technical University  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8501-6455","authenticated-orcid":false,"given":"Ilke","family":"Anac Sakir","sequence":"additional","affiliation":[{"name":"Department of Material Science and Engineering Gebze Technical University  Kocaeli Turkey"},{"name":"Institute of Biotechnology Gebze Technical University  Kocaeli Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,8,7]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"Diarrhoeal disease.World Health Organization;2020. 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