{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T01:20:44Z","timestamp":1775524844686,"version":"3.50.1"},"reference-count":45,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,10,19]],"date-time":"2021-10-19T00:00:00Z","timestamp":1634601600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study reports the increment in the secondary metabolites in <jats:italic>Stevia rebaudiana<\/jats:italic> plant after exposure to the elimination of Ca and Mg from Murashige and Skoog culture medium. The effect of nutrient stress on regenerants of <jats:italic>S. rebaudiana<\/jats:italic> is measured, which reveals significantly enhanced growth parameters, steviol glycosides (SGs) content, and nonenzymatic antioxidants; total phenolic content, total flavonoid content, total antioxidant capacity, total reducing power, and DPPH\u2010free radical scavenging activity as compared with the control treatment. However, significantly highest amounts are obtained in a medium with only Ca deficiency. The amount of rebaudioside A (Reb A) and stevioside (ST) obtained in the case of Ca\u2010deficient medium is 4.08 and 0.69%, respectively. It is followed by the results obtained from both Ca\u2010 and Mg\u2010deprived medium [Reb A (3.23%) and ST (0.52%)] and the lowest values are obtained from medium lacking Mg only [Reb A (2.60%) and ST (0.40%)]. The most probable adaptation mechanism might be the production of reactive oxygen species by nutrients\u2019 stress, which results in secondary metabolites production as defensive moieties to overcome stress situation. This effective protocol needs to be refined to apply on an industrial scale in bioreactors for increasing quantities of commercially important pharmaceutical compounds.<\/jats:p>","DOI":"10.1002\/bab.2263","type":"journal-article","created":{"date-parts":[[2021,10,5]],"date-time":"2021-10-05T07:56:36Z","timestamp":1633420596000},"page":"2008-2016","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Influence of calcium and magnesium elimination on plant biomass and secondary metabolites of <i>Stevia rebaudiana<\/i> Bertoni"],"prefix":"10.1002","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8783-1461","authenticated-orcid":false,"given":"Muhammad Arslan","family":"Ahmad","sequence":"first","affiliation":[{"name":"Shenzhen Key Laboratory of Marine Bioresource and Eco\u2010environmental Science, College of Life Sciences and Oceanography Shenzhen University  Shenzhen China"},{"name":"College of Physics and Optoelectronic Engineering Shenzhen University  Shenzhen China"},{"name":"Key Lab of Eco\u2010restoration of Regional Contaminated Environment Shenyang University, Ministry of Education  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xu","family":"Deng","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Marine Bioresource and Eco\u2010environmental Science, College of Life Sciences and Oceanography Shenzhen University  Shenzhen China"},{"name":"College of Physics and Optoelectronic Engineering Shenzhen University  Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad","family":"Adeel","sequence":"additional","affiliation":[{"name":"BNU\u2010HKUST Laboratory of Green Innovation Advanced Institute of Natural Sciences Beijing Normal University  , Tangjiawan Zhuhai Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad","family":"Rizwan","sequence":"additional","affiliation":[{"name":"College of Geography and Environmental Science Henan University  Kaifeng China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noman","family":"Shakoor","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation, College of Resources and Environmental Sciences China Agricultural University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuesuo","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Lab of Eco\u2010restoration of Regional Contaminated Environment Shenyang University, Ministry of Education  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3599-8144","authenticated-orcid":false,"given":"Rabia","family":"Javed","sequence":"additional","affiliation":[{"name":"Department of Tissue Engineering China Medical University  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,10,19]]},"reference":[{"issue":"4","key":"e_1_2_11_2_1","first-page":"178","article-title":"Micropropagation of Stevia rebaudiana Bertoni. 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