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The epidermal growth factor (EGF)\u2010functionalized LPHNPs coloaded with 5\u2010fluorouracil (FU) and sulforaphane (SFN) were prepared by one\u2010step nanoprecipitation method. Box\u2013Behnken design was applied for optimizing the material attributes and process parameters. The optimized LPHNPs revealed particle size 198\u00a0nm, polydispersity index 0.3, zeta potential<\/jats:p><jats:p>\u221225.3\u00a0mV, and drug loading efficiency 19\u201320.3% for 5\u2010FU and SFN, respectively. EGF functionalization on LPHNPs was confirmed from positive magnitude of zeta potential to 21.3\u00a0mV as compared with the plain LPHNPs. In vitro drug release performance indicated sustained and non\u2010Fickian mechanism release nature of the drugs from LPHNPs. Anticancer activity evaluation in HCT\u201015 colon cancer cells showed significant reduction (<jats:italic>p<\/jats:italic> &lt;\u00a00.001) in the cell growth and cytotoxicity of the investigated drugs from various treatments in the order: EGF\u2010functionalized LPHNPs\u00a0&gt;\u00a0plain LPHNPs\u00a0&gt;\u00a0free drug suspensions. Overall, the research work corroborated improved treatment efficacy of EGF\u2010functionalized LPHNPs for delivering chemotherapeutic agents for the management of colon carcinoma.<\/jats:p>","DOI":"10.1002\/bab.2279","type":"journal-article","created":{"date-parts":[[2021,11,14]],"date-time":"2021-11-14T08:39:58Z","timestamp":1636879198000},"page":"2205-2221","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["EGF\u2010functionalized lipid\u2013polymer hybrid nanoparticles of 5\u2010fluorouracil and sulforaphane with enhanced bioavailability and anticancer activity against colon carcinoma"],"prefix":"10.1002","volume":"69","author":[{"given":"Shumin","family":"Li","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, Binzhou Central Hospital  Binzhou Shandong Province 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