{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T14:33:32Z","timestamp":1747146812549,"version":"3.40.5"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,12,9]],"date-time":"2021-12-09T00:00:00Z","timestamp":1639008000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31560031"],"award-info":[{"award-number":["31560031"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004479","name":"Natural Science Foundation of Jiangxi Province","doi-asserted-by":"publisher","award":["20161BAB204178"],"award-info":[{"award-number":["20161BAB204178"]}],"id":[{"id":"10.13039\/501100004479","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Maltogenic amylase CoMA from <jats:italic>Corallococcus<\/jats:italic> sp. strain EGB catalyzes the hydrolysis and transglycosylation of maltooligosaccharides and soluble starch into maltose, the sole hydrolysate. This process yields pure maltose with potentially wide applications. Here, we identified and evaluated the role of phenylalanine 314 (F314), a key amino acid located near the active center, in the catalytic activities of the CoMA. Site\u2010directed mutagenesis analysis showed that the activity of a F314L mutant on potato starch substrate decreased to 26% of that of wild\u2010type protein. Compared with the wild\u2010type, F314L exhibited similar substrate specificity, hydrolysis pattern, pH, and temperature requirements. Circular dichroism spectrum data showed that the F314L mutation did not affect the structure of the folded protein. In addition, kinetic analysis demonstrated that F314L exhibited an increased <jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub> value with lower substrate affinity. Homology modeling showed that the benzene ring structure of F314L was involved in \u03c0\u2013\u03c0 conjugation, which might potentially affect the affinity of CoMA toward starch. Taken together, these data demonstrated that F314 is essential for the hydrolytic activity of the CoMA from <jats:italic>Corallococcus<\/jats:italic> sp. strain EGB.<\/jats:p>","DOI":"10.1002\/bab.2282","type":"journal-article","created":{"date-parts":[[2021,11,14]],"date-time":"2021-11-14T09:38:35Z","timestamp":1636882715000},"page":"2240-2248","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phenylalanine 314 is essential for the activity of maltogenic amylase from <i>Corallococcus<\/i> sp. EGB"],"prefix":"10.1002","volume":"69","author":[{"given":"Guorong","family":"Ni","sequence":"first","affiliation":[{"name":"Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, College of Bioscience and Bioengineering Jiangxi Agricultural University  Nanchang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingli","family":"Zhong","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Science Nanjing Agricultural University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengyao","family":"Xia","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Science Nanjing Agricultural University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lixia","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, College of Bioscience and Bioengineering Jiangxi Agricultural University  Nanchang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Longhua","family":"Dai","sequence":"additional","affiliation":[{"name":"Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, College of Bioscience and Bioengineering Jiangxi Agricultural University  Nanchang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruyi","family":"Chen","sequence":"additional","affiliation":[{"name":"Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, College of Bioscience and Bioengineering Jiangxi Agricultural University  Nanchang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuqiang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Science Nanjing Agricultural University  Nanjing China"},{"name":"Institute of Botany Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat\u2010Sen)  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8035-4786","authenticated-orcid":false,"given":"Fei","family":"wang","sequence":"additional","affiliation":[{"name":"Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, College of Bioscience and Bioengineering Jiangxi Agricultural University  Nanchang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,12,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2017.04.004"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0141-0229(01)00294-0"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcs.2019.03.018"},{"key":"e_1_2_6_5_1","first-page":"e00152","article-title":"A novel maltogenic amylase CoMA from Corallococcus sp. strain EGB catalyzes the conversion of maltooligosaccharides and soluble starch to maltose","volume":"84","author":"Jie Z","year":"2018","journal-title":"Appl. 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