{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T02:13:09Z","timestamp":1773886389275,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,12,14]],"date-time":"2021-12-14T00:00:00Z","timestamp":1639440000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Aberrant chondrocyte apoptosis and inflammation are the most critical causes of osteoarthritis (OA) development. This study was designed to demonstrate the relationship between S100A10 and OA. In this study, S100A10 was overexpressed or silenced in rat chondrocytes. Cell viability, apoptosis, reactive oxidative species (ROS), and calcium ion detection were assessed using Cell Counting Kit\u20108 assay and flow cytometry. The levels of key oxidation\u2010related enzymes and tumor necrosis factor\u2010alpha (TNF\u2010\u03b1) were quantified using enzyme\u2010linked immunosorbent assay, quantitative polymerase chain reaction, and Western blotting. S100A10 was highly expressed in patients with OA and positively correlated with TNF\u2010\u03b1 level. Knockdown of S100A10 effectively counteracted TNF\u2010\u03b1\u2010induced ROS level, apoptosis, and calcium level and associated with decreased inflammation\u2010related metalloproteinase 1 (MMP1), MMP13, and nuclear necrosis factor\u2010kappa B (NF\u2010\u03baB)\u2010p65 and increased survivin and cytoplasmic NF\u2010\u03baB\u2010p65. Overexpression of S100A10 had an effect similar to TNF\u2010\u03b1, which was significantly counteracted by pyrrolidine dithiocarbamate, an NF\u2010\u03baB inhibitor, or verapamil, a calcium\u2010channel blocker. S100A10 contributed to chondrocyte apoptosis through the ROS\/NF\u2010\u03baB pathway. This study has established the relationship between S100A10 and the NF\u2010\u03baB pathway, thus providing novel perspectives for exploring S100A10 functions.<\/jats:p>","DOI":"10.1002\/bab.2285","type":"journal-article","created":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T13:34:55Z","timestamp":1637069695000},"page":"2284-2295","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["S100A10 regulates tumor necrosis factor alpha\u2010induced apoptosis in chondrocytes via the reactive oxygen species\/nuclear factor\u2010kappa B pathway"],"prefix":"10.1002","volume":"69","author":[{"given":"Yanjie","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Orthopaedics The Second Affiliated Hospital of Xi'an Jiaotong University  Xi'an Shanxi Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruofei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics The Second Affiliated Hospital of Xi'an Jiaotong University  Xi'an Shanxi Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9632-2578","authenticated-orcid":false,"given":"Xiaoqian","family":"Dang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics The Second Affiliated Hospital of Xi'an Jiaotong University  Xi'an Shanxi Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,12,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2013.03.018"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rdc.2008.05.004"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/boneres.2016.44"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.4137\/CMAMD.S14386"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.4137\/CMAMD.S22147"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.berh.2009.08.006"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11914\u2010018\u20100477\u20101"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/1759720=12467868"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/AIA.0b013e318034194e"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/4895050"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci94753"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2011.06.020"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2010.09.006"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/284873"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2018.09.025"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20246275"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01908"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar2917"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2012.05.036"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10067\u2010008\u20101013\u2010y"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.CAN\u201011\u20101748"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2012.01.006"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2015.03.019"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3109\/15368378.2013.782316"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1089\/dna.2013.2198"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011\u2010012\u20100519\u20106"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13075\u2010017\u20101296\u2010y"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2016.01.003"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2013.08.018"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.2337\/db20\u20101157"}],"container-title":["Biotechnology and Applied Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bab.2285","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bab.2285","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bab.2285","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T04:18:12Z","timestamp":1692591492000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1002\/bab.2285"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,14]]},"references-count":30,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["10.1002\/bab.2285"],"URL":"https:\/\/doi.org\/10.1002\/bab.2285","archive":["Portico"],"relation":{},"ISSN":["0885-4513","1470-8744"],"issn-type":[{"value":"0885-4513","type":"print"},{"value":"1470-8744","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,14]]},"assertion":[{"value":"2021-08-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-12-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}