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Purified recombinant protein had molecular weight of approximately 30\u00a0kDa on 12% sodium dodecyl sulfate\u2010polyacrylamide gel electrophoresis. <jats:italic>T. hassleriana<\/jats:italic> tropinone reductase\u2010like enzyme (ThTRL) had not detected oxidative activity. The optimum pH for enzyme activity of ThTRL was weakly acidic (pH 5.0). 50\u00b0C was the optimum temperature for ThTRL. The highest catalytic efficiency and substrate affinity for recombinant ThTRL were observed with (+)\u2010camphorquinone (<jats:italic>k<\/jats:italic><jats:sub>cat<\/jats:sub>\/<jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub>\u00a0=\u00a0814.3\u00a0s<jats:sup>\u20131<\/jats:sup>\u00a0mM<jats:sup>\u20131<\/jats:sup>, <jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub>\u00a0=\u00a044.25\u00a0\u03bcM). ThTRL exhibited a broad substrate specificity and reduced various carbonyl compounds, including small lipophilic aldehydes and ketones, terpene ketones, and their structural analogs.<\/jats:p>","DOI":"10.1002\/bab.2302","type":"journal-article","created":{"date-parts":[[2021,12,14]],"date-time":"2021-12-14T00:09:50Z","timestamp":1639440590000},"page":"2530-2539","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Characterization of a putative tropinone reductase from <i>Tarenaya hassleriana<\/i> with a broad substrate specificity"],"prefix":"10.1002","volume":"69","author":[{"given":"Yixiang","family":"Li","sequence":"first","affiliation":[{"name":"The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology Jiangnan University  Wuxi Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yajun","family":"Bai","sequence":"additional","affiliation":[{"name":"College of Life Sciences Northwest University  Xi'an Shanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tai\u2010Ping","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Pharmacology University of Cambridge  Cambridge UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohui","family":"Zheng","sequence":"additional","affiliation":[{"name":"College of Life Sciences Northwest University  Xi'an Shanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9975-4085","authenticated-orcid":false,"given":"Yujie","family":"Cai","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology Jiangnan University  Wuxi Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,12,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1537-2197.1967.tb10720.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scienta.2007.03.002"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.104.049981"},{"key":"e_1_2_7_5_1","doi-asserted-by":"crossref","unstructured":"SekineT AppiahKS AziziM &FujiiY. 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