{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:03:21Z","timestamp":1791273801992,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T00:00:00Z","timestamp":1646697600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T00:00:00Z","timestamp":1646697600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Tea is a worldwide consumed herbal beverage and it was aimed in this study to reveal the major fractions of green and black tea in order to enlighten the in vitro inhibition potency on the well\u2010known drug metabolizing enzyme CYP2D6 activity. Methylxanthine fractions were extracted from green and black tea and a yield of 0.265\u00a0g (1.06%) for 25\u00a0g of dried black tea and 0.302\u00a0g (1.2%) for 25\u00a0g of green tea was calculated. High\u2010performance liquid chromatography analysis represented that the major components of the methylxanthine fractions were caffeine, theobromine, and theophylline. Methylxanthine content of black tea was 368.25\u00a0\u00b1\u00a04.6\u00a0\u03bcg\/ml caffeine, 89.30\u00a0\u00b1\u00a02.3\u00a0\u03bcg\/ml theobromine, and 3.40\u00a0\u00b1\u00a00.5\u00a0\u03bcg\/ml theophylline, whereas that of green tea was 176.50\u00a0\u00b1\u00a03.7\u00a0\u03bcg\/ml caffeine, 53.85\u00a0\u00b1\u00a01.4\u00a0\u03bcg\/ml theobromine, and 2.06\u00a0\u00b1\u00a00.7 \u03bcg\/ml theophylline. The results of concentration\u2010dependent inhibition studies were 76% green tea, 75% black tea, and 55% caffeine at concentration of 10\u00a0mg\/ml. The inhibition rates of green and black tea on CYP2D6 activity were 76% and 75%, respectively, where that of quinidine, the well\u2010known inhibitor of CYP2D6, was 82%. Our results indicate that green and black tea is very likely to modify the CYP2D6 enzyme activity.<\/jats:p>","DOI":"10.1002\/bab.2325","type":"journal-article","created":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T17:53:20Z","timestamp":1645638800000},"page":"22-27","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The analysis of methylxanthine fractions obtained from\n                    <i>Camellia sinensis<\/i>\n                    cultivated in Turkey and effects on the in vitro inhibition of CYP2D6 enzyme"],"prefix":"10.1002","volume":"70","author":[{"given":"Azade","family":"Attar","sequence":"first","affiliation":[{"name":"Faculty of Chemical &amp; Metallurgical Engineering, Department of Bioengineering Yildiz Technical University  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0800-1249","authenticated-orcid":false,"given":"Melda","family":"Altikatoglu Yapaoz","sequence":"additional","affiliation":[{"name":"Faculty of Science and Letters, Department of Chemistry Yildiz Technical University  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,3,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)49939-X"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389200221666200512112718"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2016.10.055"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics13020148"},{"key":"e_1_2_6_6_1","doi-asserted-by":"crossref","unstructured":"FeltrinC FariasIV SandjoLP ReginattoFH CuMOJCRiT Sim\u00f5e: Effects of standardized medicinal plant extracts on drug metabolism mediated by CYP3A4 and CYP2D6 enzymes.2020;33(9):2408\u201019.","DOI":"10.1021\/acs.chemrestox.0c00182"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","unstructured":"HellumBH HuZ NilsenOGJB pharmacology c toxicology: The induction of CYP1A2 CYP2D6 and CYP3A4 by six trade herbal products in cultured primary human hepatocytes.2007;100(1):23\u201030.","DOI":"10.1111\/j.1742-7843.2007.00011.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2010.11.009"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2013.49"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2014.02.014"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.5308"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2017.09.023"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0043-100934"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.6063"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.8b05140"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2009.09.022"},{"key":"e_1_2_6_17_1","doi-asserted-by":"crossref","unstructured":"Flisz\u00e1r\u2010Ny\u00falE MohosV CsepregiR Mlad\u011bnkaP Po\u00f3rMJB Pharmacotherapy: Inhibitory effects of polyphenols and their colonic metabolites on CYP2D6 enzyme using two different substrates.2020;131:110732.","DOI":"10.1016\/j.biopha.2020.110732"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.10.064"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20112681"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-973836"},{"issue":"7","key":"e_1_2_6_21_1","first-page":"591","article-title":"Green tea: potential health benefits","volume":"79","author":"Schneider C","year":"2009","journal-title":"Am Fam Physician"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.29252\/nfsr.7.2.37"},{"key":"e_1_2_6_23_1","unstructured":"GjlsB: \u0130la\u00e7lar \u0130le Teofilin Teobromin Kafein \u0130\u00e7eren Besinler Arasindaki Etkile\u015fimler. 2018;2(2):82\u201096."},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-50468-7"},{"key":"e_1_2_6_25_1","doi-asserted-by":"crossref","unstructured":"PengL SongX ShiX LiJ YeC PengLi SongXiaohong ShiXianggang LiJiaxian YeChuangxing PengLi SongXiaohong ShiXianggang LiJiaxian&YeChuangxing: An improved HPLC method for simultaneous determination of phenolic compounds purine alkaloids and theanine in Camellia species. 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