{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T21:01:54Z","timestamp":1785445314999,"version":"3.56.0"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,5,9]],"date-time":"2022-05-09T00:00:00Z","timestamp":1652054400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2022,5,9]],"date-time":"2022-05-09T00:00:00Z","timestamp":1652054400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Based on the broad clinical utility of the immunosuppressant mycophenolic acid (MPA), this article aims to intensify the biosynthetic potential of\n                    <jats:italic>Penicillium roqueforti<\/jats:italic>\n                    for more effective hyperproduction of the drug. Several mutants were generated from irradiation mutagenesis and screened. Two strains (GM1013 and GM1093) presented an elevated MPA productivity with significant yield constancy over 10 subsequent generations. By investigating the effect of some phosphorous sources and mineral salts on MPA production by the two mutants, KH\n                    <jats:sub>2<\/jats:sub>\n                    PO\n                    <jats:sub>4<\/jats:sub>\n                    and FeSO\n                    <jats:sub>4<\/jats:sub>\n                    \u00b77H\n                    <jats:sub>2<\/jats:sub>\n                    O were most preferred by the two mutants for higher MPA production rates. Statistics\u2010dependent experimental designs were also employed for optimizing medium components for maximum MPA production. Medium components were primarily screened using the Plackett\u2013Burman model to demonstrate the most important components that most significantly affect MPA production. The concentrations of these significant components were then optimized through a central composite rotatable model. In conclusion, gamma\u2010radiation mutation and response surface optimization resulted in a promising MPA productivity by\n                    <jats:italic>P. roqueforti<\/jats:italic>\n                    GM1013. To our knowledge, the MPA\u2010yield achieved in this study (2933.32\u00a0mg\u00a0L\n                    <jats:sup>\u20131<\/jats:sup>\n                    ) is the highest reported by academic laboratories from\n                    <jats:italic>P. roqueforti<\/jats:italic>\n                    cultures, which could be of economic value for a prospective large industrialized application.\n                  <\/jats:p>","DOI":"10.1002\/bab.2353","type":"journal-article","created":{"date-parts":[[2022,4,28]],"date-time":"2022-04-28T06:29:46Z","timestamp":1651127386000},"page":"306-317","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Unlocking the biosynthetic potential of\n                    <i>Penicillium roqueforti<\/i>\n                    for hyperproduction of the immunosuppressant mycophenolic acid: Gamma radiation mutagenesis and response surface optimization of fermentation medium"],"prefix":"10.1002","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7867-3801","authenticated-orcid":false,"given":"El\u2010Sayed R.","family":"El\u2010Sayed","sequence":"first","affiliation":[{"name":"Plant Research Department, Nuclear Research Center Egyptian Atomic Energy Authority  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5024-663X","authenticated-orcid":false,"given":"Amira G.","family":"Zaki","sequence":"additional","affiliation":[{"name":"Plant Research Department, Nuclear Research Center Egyptian Atomic Energy Authority  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,5,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/ctr.13997"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1821932116"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0193749"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2014.12.023"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.4081\/ijfs.2014.2118"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13213\u2010019\u201001517\u2010z"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.btre.2016.07.003"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12010\u2010016\u20102313\u20103"},{"key":"e_1_2_8_10_1","volume-title":"Bioreactor engineering research and industrial applications I. Advances in biochemical engineering and biotechnology","author":"Qin H","year":"2015"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/10_2008_26"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274\u2010014\u20101685\u20101"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jam.12828"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcab.2019.01.053"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13568-022-01386-x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjbs.2021.12.019"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284\u2010020\u201001874\u2010y"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.1.550\u2010553.2005"},{"key":"e_1_2_8_19_1","unstructured":"QueenerSW NashCH.Procedure for obtainingPenicilliumspecies mutants with improved ability to synthesize mycophenolic acid. 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