{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T02:12:04Z","timestamp":1768702324145,"version":"3.49.0"},"reference-count":71,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,8,8]],"date-time":"2022-08-08T00:00:00Z","timestamp":1659916800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001843","name":"Science and Engineering Research Board","doi-asserted-by":"publisher","award":["EMR\/2016\/001183"],"award-info":[{"award-number":["EMR\/2016\/001183"]}],"id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Leptospirosis is a tropical and globally neglected zoonotic disease caused by pathogenic spirochetes, <jats:italic>Leptospira<\/jats:italic>. Although the disease has been studied for decades, a potent or effective vaccine is not available so far. Efforts are being made to design an efficient vaccine candidate using different approaches. Immunoinformatics approaches have been proven to be promising in terms of time and cost. Here, we used immunoinformatics and structure\u2010based approaches to evaluate antigenic B\u2010 and T\u2010cell epitopes present on the leptospiral lipoproteins (LipL). The promiscuous overlapping epitopes (B\u2010cell, T\u2010cell, interferon (IFN)\u2010\u03b3 positive, and non\u2010allergens), which can induce humoral, cell\u2010mediated, and innate immunity, were selected to generate a multiepitope chimeric vaccine. To enhance the vaccine immunogenicity, a Toll\u2010like receptor (TLR) agonist was fused to the vaccine with a suitable linker. The chimeric vaccine structure was predicted for molecular docking studies with immune receptors. Moreover, the stability of the vaccine\u2013immune receptor complexes was analyzed by normal mode analysis (NMA). The potency of the vaccine construct was predicted by the immune simulation tool. The study provides additional information toward constructing peptide\u2010based chimeric vaccines \u00a0against <jats:italic>Leptospira<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/bab.2389","type":"journal-article","created":{"date-parts":[[2022,7,25]],"date-time":"2022-07-25T18:54:18Z","timestamp":1658775258000},"page":"670-687","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Multiepitope\u2010based vaccine design by exploring antigenic potential among leptospiral lipoproteins using comprehensive immunoinformatics and structure\u2010based approaches"],"prefix":"10.1002","volume":"70","author":[{"given":"Pankaj","family":"Kumar","sequence":"first","affiliation":[{"name":"Laboratory of Structural Biology, Department of Biochemistry School of Life Sciences, University of Hyderabad  Gachibowli Hyderabad Telangana 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