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There has been a considerable interest in the improvement of spinosad production because of a low yield achieved by wild\u2010type\n                    <jats:italic>S. spinosa<\/jats:italic>\n                    . In this study, we designed and constructed a pIBR\u2010SPN vector. pIBR\u2010SPN is an integrative vector that can be used to introduce foreign genes into the chromosome of\n                    <jats:italic>S. spinosa<\/jats:italic>\n                    . Different combinations of genes encoding forasamine and rhamnose were synthesized and used for the construction of different recombinant plasmids. The following recombinant strains were developed:\n                    <jats:italic>S. spinosa<\/jats:italic>\n                    pIBR\u2010SPN (only the vector),\n                    <jats:italic>S. spinosa<\/jats:italic>\n                    pIBR\u2010SPN F (forosamine genes),\n                    <jats:italic>S. spinosa<\/jats:italic>\n                    pIBR\u2010SPN R (rhamnose genes),\n                    <jats:italic>S. spinosa<\/jats:italic>\n                    pIBR\u2010SPN FR (forosamine and rhamnose genes),\n                    <jats:italic>S. spinosa<\/jats:italic>\n                    pIBR\u2010SPN FRS (forosamine, rhamnose, and SAM [S\u2010adenosyl\u2010L\u2010methionine synthetase] genes), and\n                    <jats:italic>S. spinosa<\/jats:italic>\n                    MUV pIBR\u2010SPN FR. Among these recombinant strains,\n                    <jats:italic>S. spinosa<\/jats:italic>\n                    pIBR\u2010SPN FR produced 1394 \u00b1 163\u00a0mg\/L spinosad, which was 13\u2010fold higher than the wild\u2010type.\n                    <jats:italic>S. spinosa<\/jats:italic>\n                    MUV pIBR\u2010SPN FR produced 1897 (\u00b1129)\u00a0mg\/L spinosad, which was seven\u2010fold higher than\n                    <jats:italic>S. spinosa<\/jats:italic>\n                    MUV and 17\u2010fold higher than the wild\u2010type strain.\n                  <\/jats:p>","DOI":"10.1002\/bab.2418","type":"journal-article","created":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T16:13:45Z","timestamp":1670429625000},"page":"1035-1043","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Increasing production of spinosad in\n                    <i>Saccharopolyspora spinosa<\/i>\n                    by metabolic engineering"],"prefix":"10.1002","volume":"70","author":[{"given":"Adzemye Fovennso","family":"Bridget","sequence":"first","affiliation":[{"name":"Institute of Biomolecule Reconstruction (iBR), Department of Life Science and Biochemical Engineering Sun Moon University  Asan\u2010si Chungnam Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chung Thanh","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Institute of Biomolecule Reconstruction (iBR), Department of Life Science and Biochemical Engineering Sun Moon University  Asan\u2010si Chungnam Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rubin Thapa","family":"Magar","sequence":"additional","affiliation":[{"name":"Institute of Biomolecule Reconstruction (iBR), Department of Life Science and Biochemical Engineering Sun Moon University  Asan\u2010si Chungnam 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