{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T02:38:51Z","timestamp":1792463931323,"version":"4.5.0"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The purpose of this article is to design a green and comprehensive utilization process for preparing chitosan from crab shells. Glutamate acid was used as a decalcifying agent for crab shells, and the mixed solution of potassium hydroxide\/isopropanol was used for deproteinization and deacetylation to prepare chitosan. Glutamic acid and isopropanol could be recovered for recycling. At the same time, calcium carbonate and protein in crab shells were converted into calcium hydrogen phosphate and compound fertilizer containing nitrogen, phosphorus, and potassium, respectively. The prepared chitosan was characterized by Fourier\u2010transform infrared (FT\u2010IR), differential scanning calorimetry (DSC), x\u2010ray diffraction (XRD), and scanning electron microscopy (SEM), and its deacetylation degree and viscosity average molecular weight were 88.7%\u00a0\u00b1\u00a00.68% and 792.1\u00a0\u00b1\u00a010.82\u00a0kDa, respectively. The recoveries of glutamic acid and isopropanol were 95.56%\u00a0\u00b1\u00a01.39% and 88.14%\u00a0\u00b1\u00a01.13%, respectively. The prepared chitosan has large molecular weight and deacetylation degree, controllable production cost, comprehensive utilization of crab shell components, and greatly reduced waste emissions.<\/jats:p>","DOI":"10.1002\/bab.2450","type":"journal-article","created":{"date-parts":[[2023,2,17]],"date-time":"2023-02-17T19:14:42Z","timestamp":1676661282000},"page":"1421-1434","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Design and experimental study on closed\u2010loop process of preparing chitosan from crab shells"],"prefix":"10.1002","volume":"70","author":[{"given":"Huiming","family":"Wang","sequence":"first","affiliation":[{"name":"College of Biology and Environment Zhejiang Wanli University  Ningbo Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huien","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Biology and Environment Zhejiang Wanli University  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