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Here we investigated the abilities of our isolates from diverse conventional sources to characterize the beneficial bacteria for inhibition of <jats:italic>S. mutans<\/jats:italic>. <jats:italic>Enterobacter cloacae<\/jats:italic> PS\u201074, a beneficial bacteria isolated from yoghurt, is gram negative, rod shaped, and resistant to acid, bile salt, and amylase. PS\u201074 cell\u2010free supernatants (CFS) demonstrated highest zone of inhibition of 29\u00a0\u00b1\u00a01.7\u00a0mm. Further, the minimum inhibitory concentration (MIC) value of CFS PS\u201074 was recorded to be 10\u00a0\u03bcL and its minimum bactericidal concentration (MBC) value was found to be 15\u03bcL which led to 99.9% log reduction of <jats:italic>S. mutans<\/jats:italic>. Moreover, the biofilm formation was reduced by 84.91% at MIC15 of CFS PS\u201074 which alleviate the dental caries formation by <jats:italic>S. mutans<\/jats:italic>. This is the first report on <jats:italic>E. cloacae<\/jats:italic> PS\u201074, which was studied for its probiotic properties to inhibit <jats:italic>S. mutans<\/jats:italic> MTCC\u2010890 due to the production of organic acids and employed in oral treatment.<\/jats:p>","DOI":"10.1002\/bab.2471","type":"journal-article","created":{"date-parts":[[2023,5,26]],"date-time":"2023-05-26T06:06:47Z","timestamp":1685081207000},"page":"1518-1529","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Alleviation of dental caries by use of isolated potential probiotic and its characterization"],"prefix":"10.1002","volume":"70","author":[{"given":"Preeti","family":"Pallavi","sequence":"first","affiliation":[{"name":"Center for Biotechnology, School of Pharmaceutical Sciences Siksha \u2018O\u2019 Anusandhan Deemed to be University  Bhubaneswar Odisha 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