{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T19:09:34Z","timestamp":1779908974048,"version":"3.53.1"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,6,27]],"date-time":"2023-06-27T00:00:00Z","timestamp":1687824000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005001","name":"Ahvaz Jundishapur University of Medical Sciences","doi-asserted-by":"publisher","award":["N\u20109712"],"award-info":[{"award-number":["N\u20109712"]}],"id":[{"id":"10.13039\/501100005001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Reliable and precise quantification of 25\u2010hydroxyvitamin D<jats:sub>3<\/jats:sub> in clinical samples is vital because vitamin D<jats:sub>3<\/jats:sub> deficiency lead to several disorders, such as mental illness, osteoporosis, and coronavirus disease. Herein, we report the fabrication of a novel electrochemical aptasensor using a nanocomposite, including reduced graphene oxide, pyrrole, and <jats:sc>l<\/jats:sc>\u2010cysteine, for the sensitive detection of 25\u2010hydroxyvitamin D<jats:sub>3<\/jats:sub>. Subsequently, the aptamer of 25\u2010hydroxyvitamin D<jats:sub>3<\/jats:sub> was immobilized on the surface of the modified electrode. Differential pulse voltammetry signals were utilized for studying the binding and measurement of 25\u2010hydroxyvitamin D<jats:sub>3<\/jats:sub> based on the oxidation peak. Under the optimum conditions, the designed electrochemical aptasensor exhibited a linear detection range of 0.001\u2013150\u00a0nM, with a limit of detection of 0.006\u00a0nM. Furthermore, the proposed aptasensor selectively detected 25\u2010hydroxyvitamin D<jats:sub>3<\/jats:sub> compared to other analogs. Moreover, this aptasensor was successfully applied for the detection of 25\u2010hydroxyvitamin D<jats:sub>3<\/jats:sub> in human serum samples, which were quantified by the enzyme\u2010linked immunosorbent assay method. The acceptable recoveries of 82.67%\u2013111.07% demonstrated that this proposed electrochemical aptasensor can be a promising alternative for clinical methods of vitamin D determination.<\/jats:p>","DOI":"10.1002\/bab.2490","type":"journal-article","created":{"date-parts":[[2023,6,27]],"date-time":"2023-06-27T08:09:22Z","timestamp":1687853362000},"page":"1881-1894","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Designing a label\u2010free electrochemical aptasensor based on polypyrrole\u2010<scp>l<\/scp>\u2010cysteine\u2010reduced graphene oxide nanocomposite for detection of 25\u2010hydroxyvitamin D<sub>3<\/sub>"],"prefix":"10.1002","volume":"70","author":[{"given":"Maryam","family":"Heydari","sequence":"first","affiliation":[{"name":"Nanotechnology Research Center Ahvaz Jundishapur University of Medical Sciences Ahvaz 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