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In continuation of our previous study on the identification of BH4 from cyanide\u2010degrading <jats:italic>Bacillus pumilus<\/jats:italic>, the present study focuses on evaluating the anticancer properties of BH4 on A549, a human lung adenocarcinoma. Anticancer activity analysis shows that BH4 inhibited A549 cell growth after 24\u00a0h of incubation with 0.02\u00a0mg\/mL. In acridine orange\/ethidium bromide staining, BH4\u2010treated A549 cells showed apoptotic morphology. BH4 treatment caused cell cycle arrest at G0\/G1 phase compared to control cells. BH4 augmented p53 expression in alveolar cancer cells by downregulating MDM2 levels. There was downregulation of casp\u20103 and upregulation of iNOS gene in BH4\u2010treated A549 cells. Further, docking studies indicated that BH4 had significant interactions with the above proteins affirming the apoptosis mechanism. 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