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In the current study, the bio\u2010accessibility of <jats:italic>H. persicum<\/jats:italic> bioactive compounds was improved by purifying its phenolic\u2010enriched fractions (PEF) and encapsulating them into nanoliposomes to analyze its cytotoxic impacts on mice testicular tissue and their fertility status. Nano liposomal <jats:italic>H. persicum<\/jats:italic> PEF (NL\u2010HPEF) was prepared by ultrasound\u2010based encapsulation of HPEF and L\u2010agranular lecithin mixture. The size, morphology, and stability of NL\u2010HPEF were characterized by dynamic light scattering, field emission scanning electron microscopy, and zeta potential analysis. The 18 white male Balb\/c mice (20\u201325\u00a0g) at 3 treatment groups were provided to study the NL\u2010HPPF cytotoxicity by measuring the mice liver enzyme including aspartate aminotransferase (AST), ALP and alanine aminotransferase (ALT), testis lipid peroxidation, and testicular tissue destruction levels. Moreover, the mice's fertility was evaluated by studying the Adam3, Prm1, Spata19, and Tnp2 gene expression in the testicular tissues. The obtained results manifested that the synthesized NL\u2010HPEF was stable (193.7\u00a0nm) and exhibited a notable cytotoxic impact on the mice's liver (ALT and AST enhancement levels) and testicular tissues. Moreover, their increasing treatment doses impaired the male mice's fertility by decreasing the sperm count, viability, and motility. In addition, fertility suppression was verified by decreasing serum testosterone and downregulating the Adam3, Prm1, Spata19, and Tnp2 gene expression in their testicular tissues. The male mice's fertility was significantly (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05) suppressed by increasing treatment doses of NL\u2010HPEF. Hence, the NL\u2010HPEF could be considered a promising alternative to replace the male chemical contraceptives drugs.<\/jats:p>","DOI":"10.1002\/bab.2511","type":"journal-article","created":{"date-parts":[[2023,9,11]],"date-time":"2023-09-11T09:40:19Z","timestamp":1694425219000},"page":"2088-2096","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The anti\u2010spermatogenic activity of nanoliposomes loaded with <i>Heracleum persicum<\/i> phenolic compounds in Balb\/C mice"],"prefix":"10.1002","volume":"70","author":[{"given":"Seyed Amirsajjad","family":"Alami","sequence":"first","affiliation":[{"name":"Department of Biology, Mashhad Branch Islamic Azad University  Mashhad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aryan","family":"Payrovnaziri","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical, Chemical and Environmental Sciences, Faculty of Engineering and Science University of Greenwich, Medway  Chatham Maritime Kent UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saeed","family":"Seghatoleslami","sequence":"additional","affiliation":[{"name":"Department of Biology, Mashhad Branch Islamic Azad University  Mashhad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Faraji","sequence":"additional","affiliation":[{"name":"Department of Biology, Mashhad Branch Islamic Azad University  Mashhad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatemeh Rahimzadeh","family":"Bajgiran","sequence":"additional","affiliation":[{"name":"Department of Biology, Mashhad Branch Islamic Azad University  Mashhad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahsa Rastegar Moghaddam","family":"Poorbagher","sequence":"additional","affiliation":[{"name":"Department of Biology, Mashhad Branch Islamic Azad University  Mashhad 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