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RhB attaches to the GO surface via electrostatic attraction, leading to the quenching of its fluorescence upon the establishment of the RhB\u2013GO complex. However, the presence of GAGs prompts a resurgence of intense fluorescence. The linear range of the method is between 5.00 and 70.00\u00a0mg\/L. The total GAG levels of urine samples analyzed using the method agree with the results of the biochemistry analysis laboratory (65.85 and 79.18\u00a0mg\/L; 73.30\u00a0\u00b1 1.76 and 72.21\u00a0\u00b1 2.21). The method is simple, accurate, and sensitive and may be used for both first\u2010step diagnosis of the mucopolysaccharidoses and detection of individual GAGs for studies of GAG\u2010related research and other biological applications.<\/jats:p>","DOI":"10.1002\/bab.2565","type":"journal-article","created":{"date-parts":[[2024,3,7]],"date-time":"2024-03-07T05:03:30Z","timestamp":1709787810000},"page":"651-660","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A novel graphene oxide\u2010based fluorescence method for detection of urine glycosaminoglycans"],"prefix":"10.1002","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-6802-9174","authenticated-orcid":false,"given":"Ceren Bak\u0131r","family":"K\u00f6so\u011flu","sequence":"first","affiliation":[{"name":"Department of Chemistry, Faculty of Arts and Sciences Yildiz Technical University  Esenler Istanbul Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3342-4112","authenticated-orcid":false,"given":"S\u00fcreyya","family":"Dede","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Arts and Sciences Yildiz Technical University  Esenler Istanbul Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7730-3304","authenticated-orcid":false,"given":"Emine","family":"Karaku\u015f","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Arts and Sciences Yildiz Technical University  Esenler Istanbul Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9282-7651","authenticated-orcid":false,"given":"Ali","family":"Erdo\u011fmu\u015f","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Arts and Sciences Yildiz Technical University  Esenler Istanbul Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8502-8982","authenticated-orcid":false,"given":"Bahad\u0131r","family":"Keskin","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Arts and Sciences Yildiz Technical University  Esenler Istanbul Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9676-7086","authenticated-orcid":false,"given":"Hasan","family":"\u00d6nal","sequence":"additional","affiliation":[{"name":"Pediatric Endocrinology and Metabolic Diseases Clinic University of Health Sciences, Basaksehir Cam and Sakura City Hospital  Basaksehir Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,3,6]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1747-0285.2008.00741.x"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.6.9.1612287"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-018-2953-y"},{"key":"e_1_2_11_5_1","volume-title":"Biochemistry, glycosaminoglycans. 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