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In this study, we assessed the efficacy of bacoside\u2010A (a dammarane\u2010type triterpenoid saponin derived from the plant <jats:italic>Bacopa monniera<\/jats:italic> Linn.) on the adipogenesis of 3T3\u2010L1 preadipocytes. Results of this study illustrated that bacoside\u2010A decreased the differentiation of 3T3\u2010L1 cell, as evidenced by diminution of lipid droplets, which contains triglycerides and other lipids. During the differentiation process, transcription factors, which are mainly participating in adipogenesis such us CCAAT\/enhancer\u2010binding protein \u03b1 (C\/EBP\u03b1) and C\/EBP\u03b2, peroxisome proliferator\u2010activated receptor\u2010\u03b3 (PPAR\u03b3), and sterol regulatory element\u2010binding protein\u20101c (SREBP\u20101c), expressions were significantly suppressed by bacoside\u2010A. In addition, bacoside\u2010A showed a potent reduction in genes precise to adipocytes such as lipoprotein lipase (LPL), fatty acid synthase (FAS), adipocyte fatty acid\u2010binding protein (FABP4), and leptin expressions. Further, bacoside\u2010A stimulated the phosphorylation of acetyl CoA carboxylase (ACC) and AMP\u2010activated protein kinase (AMPK). These results demonstrated that bacoside\u2010A has anti\u2010adipogenic effects by regulating the transcription factors involved in adipocyte differentiation. Therefore, bacoside\u2010A might be considered as a potent therapeutic agent for alleviating obesity and hyperlipidemia.<\/jats:p>","DOI":"10.1002\/bab.2573","type":"journal-article","created":{"date-parts":[[2024,2,29]],"date-time":"2024-02-29T06:53:52Z","timestamp":1709189632000},"page":"741-752","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Bacoside\u2010A repressed the differentiation and lipid accumulation of 3T3\u2010L1 preadipocytes by modulating the expression of adipogenic genes"],"prefix":"10.1002","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6478-0833","authenticated-orcid":false,"given":"Thiyagarajan","family":"Ramesh","sequence":"first","affiliation":[{"name":"Department of Basic Medical Sciences College of Medicine Prince Sattam Bin Abdulaziz University  Al\u2010Kharj Kingdom of Saudi 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