{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T14:34:13Z","timestamp":1747146853700,"version":"3.40.5"},"reference-count":52,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,3,21]],"date-time":"2024-03-21T00:00:00Z","timestamp":1710979200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004484","name":"Tehran University of Medical Sciences and Health Services","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004484","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sepsis is a potentially fatal syndrome related to severe systemic inflammation developed by infection. Despite different antimicrobial therapies, morbidity and mortality rates remain high. Herbs along with cell therapy have been introduced as a promising option to improve the symptoms of sepsis. The present study aimed to evaluate the therapeutic effect of simultaneous administration of thyme essential oil (TEO) and endothelial progenitor stem cells (EPCs) on lipopolysaccharide (LPS)\u2010induced sepsis in C57BL\/6 mice. Sepsis was induced in C57Bl\/6J mice by intraperitoneal injection of LPS, followed 2\u00a0h later by an intravenous injection of EPCs or oral administration of TEO or simultaneous administration of TEO and EPCs. After 10 days, the complete blood cell, renal and liver factors, serum levels of inflammatory cytokines, and angiogenic factors were measured. Simultaneous treatment with EPCs and TEO significantly increased the survival of mice with sepsis and modulated the inflammatory response by reducing the serum levels of pro\u2010inflammatory cytokines. Moreover, this treatment significantly reduced the level of white blood cells and neutrophils and increased the number of red blood cells, the percentage of hematocrit, and hemoglobin. The combination of TEO with EPCs decreased organ injuries and was assessed by lower levels of the liver enzymes alanine aminotransferase and aspartate aminotransferase compared to the sepsis group. Administration of EPCs and TEO also significantly improved angiogenic factors, lung function, and toll\u2010like receptor 4 expression. EPCs in combination with TEO increase survival in the LPS\u2010induced sepsis mice model by acting on several targets. Thus, the combination of TEO with EPCs can be a feasible approach for the future clinical treatment and control of sepsis.<\/jats:p>","DOI":"10.1002\/bab.2580","type":"journal-article","created":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T06:33:34Z","timestamp":1711089214000},"page":"835-848","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The effect of thyme essential oil and endothelial progenitor stem cells on lipopolysaccharide\u2010induced sepsis in C57BL\/6 mice"],"prefix":"10.1002","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3421-3974","authenticated-orcid":false,"given":"Mohammad Mehdi","family":"Soltan Dallal","sequence":"first","affiliation":[{"name":"Food Microbiology Research Center Tehran University of Medical Sciences  Tehran Iran"},{"name":"Department of Pathobiology, School of Public Health Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryam","family":"Siavashi","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, School of Public Health Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samira","family":"Karimaei","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, School of Public Health Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vahid","family":"Siavashi","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, Faculty of Veterinary Medicine University of Tehran  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milad","family":"Abdi","sequence":"additional","affiliation":[{"name":"Research Center of Tropical and Infectious Diseases Kerman University of Medical Sciences  Kerman Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehdi","family":"Yaseri","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Biostatistics, School of Public Health Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seyed Alireza","family":"Razavi","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, School of Public Health Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronak","family":"Bakhtiari","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, School of Public Health Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,3,21]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_11_2_1","DOI":"10.1016\/S0140-6736(10)60564-8"},{"doi-asserted-by":"publisher","key":"e_1_2_11_3_1","DOI":"10.1159\/isbn.978-3-8055-9485-1"},{"doi-asserted-by":"publisher","key":"e_1_2_11_4_1","DOI":"10.2131\/jts.43.329"},{"doi-asserted-by":"publisher","key":"e_1_2_11_5_1","DOI":"10.1097\/CCM.0000000000000026"},{"doi-asserted-by":"publisher","key":"e_1_2_11_6_1","DOI":"10.1164\/rccm.201504-0781OC"},{"doi-asserted-by":"publisher","key":"e_1_2_11_7_1","DOI":"10.1097\/CCM.0000000000002255"},{"doi-asserted-by":"publisher","key":"e_1_2_11_8_1","DOI":"10.1016\/j.molmed.2014.01.007"},{"doi-asserted-by":"publisher","key":"e_1_2_11_9_1","DOI":"10.1186\/1742-2094-11-25"},{"issue":"4","key":"e_1_2_11_10_1","first-page":"271","article-title":"Vitamins, omega\u20103, magnesium, manganese, and thyme can boost our immunity and protect against COVID\u201019","volume":"10","author":"Hamada AM","year":"2020","journal-title":"Eur J Biol Res"},{"doi-asserted-by":"publisher","key":"e_1_2_11_11_1","DOI":"10.1016\/j.cbi.2023.110426"},{"doi-asserted-by":"publisher","key":"e_1_2_11_12_1","DOI":"10.3390\/molecules25184125"},{"doi-asserted-by":"publisher","key":"e_1_2_11_13_1","DOI":"10.1186\/s13287-015-0192-0"},{"doi-asserted-by":"publisher","key":"e_1_2_11_14_1","DOI":"10.1002\/stem.544"},{"doi-asserted-by":"publisher","key":"e_1_2_11_15_1","DOI":"10.3390\/ijms17071164"},{"doi-asserted-by":"publisher","key":"e_1_2_11_16_1","DOI":"10.4252\/wjsc.v7.i2.368"},{"doi-asserted-by":"publisher","key":"e_1_2_11_17_1","DOI":"10.1128\/ecosalplus.esp-0019-2022"},{"doi-asserted-by":"publisher","key":"e_1_2_11_18_1","DOI":"10.21769\/BioProtoc.4227"},{"doi-asserted-by":"publisher","key":"e_1_2_11_19_1","DOI":"10.2147\/COPD.S110781"},{"doi-asserted-by":"publisher","key":"e_1_2_11_20_1","DOI":"10.1007\/s12015-022-10432-x"},{"doi-asserted-by":"publisher","key":"e_1_2_11_21_1","DOI":"10.1159\/000324758"},{"doi-asserted-by":"publisher","key":"e_1_2_11_22_1","DOI":"10.7150\/ijbs.74456"},{"issue":"2","key":"e_1_2_11_23_1","first-page":"10","article-title":"Antimicrobial effect of Zataria multiflora Boiss essential oil on Escherichia coli O157:H7 during manufacture and ripening of white brined cheese","volume":"1","author":"Mohammadi KH","year":"2011","journal-title":"J Food Hyg"},{"issue":"1","key":"e_1_2_11_24_1","first-page":"105","article-title":"Catalytic oxidation and antioxidant properties of thyme essential oils (Thymus vulgarae L.)","volume":"78","author":"Juki\u0107 M","year":"2005","journal-title":"Croat Chem Acta"},{"doi-asserted-by":"publisher","key":"e_1_2_11_25_1","DOI":"10.30699\/jambs.29.133.83"},{"doi-asserted-by":"publisher","key":"e_1_2_11_26_1","DOI":"10.4103\/japtr.JAPTR_366_18"},{"doi-asserted-by":"publisher","key":"e_1_2_11_27_1","DOI":"10.1016\/j.jff.2021.104436"},{"doi-asserted-by":"publisher","key":"e_1_2_11_28_1","DOI":"10.1016\/j.ejphar.2018.06.003"},{"doi-asserted-by":"publisher","key":"e_1_2_11_29_1","DOI":"10.1136\/gut.2008.168534"},{"doi-asserted-by":"publisher","key":"e_1_2_11_30_1","DOI":"10.1097\/SHK.0000000000000039"},{"doi-asserted-by":"publisher","key":"e_1_2_11_31_1","DOI":"10.1002\/jcb.25981"},{"doi-asserted-by":"publisher","key":"e_1_2_11_32_1","DOI":"10.3390\/jcm7110400"},{"doi-asserted-by":"publisher","key":"e_1_2_11_33_1","DOI":"10.1186\/s13287-020-01858-6"},{"doi-asserted-by":"publisher","key":"e_1_2_11_34_1","DOI":"10.1371\/journal.pone.0020219"},{"doi-asserted-by":"publisher","key":"e_1_2_11_35_1","DOI":"10.1038\/srep31528"},{"doi-asserted-by":"publisher","key":"e_1_2_11_36_1","DOI":"10.4252\/wjsc.v6.i2.120"},{"issue":"3","key":"e_1_2_11_37_1","first-page":"161","article-title":"Cytokines in the systemic inflammatory response syndrome: a review","volume":"2","author":"Jaffer U","year":"2010","journal-title":"HSR Proc Intensive Care Cardiovasc Anesth"},{"doi-asserted-by":"publisher","key":"e_1_2_11_38_1","DOI":"10.1378\/chest.107.4.1062"},{"doi-asserted-by":"publisher","key":"e_1_2_11_39_1","DOI":"10.1002\/jcp.28077"},{"doi-asserted-by":"publisher","key":"e_1_2_11_40_1","DOI":"10.1038\/nm.1905"},{"doi-asserted-by":"publisher","key":"e_1_2_11_41_1","DOI":"10.1186\/1471-2172-12-55"},{"doi-asserted-by":"publisher","key":"e_1_2_11_42_1","DOI":"10.1186\/s13054-015-0778-z"},{"doi-asserted-by":"publisher","key":"e_1_2_11_43_1","DOI":"10.1002\/stem.1270"},{"issue":"3","key":"e_1_2_11_44_1","first-page":"1","article-title":"Toll\u2010like receptor 4 in phagocytosis of Escherichia coli by endotoxin\u2010activated human neutrophils in whole blood","volume":"16","author":"Prokhorenko I","year":"2012","journal-title":"Crit Care"},{"doi-asserted-by":"publisher","key":"e_1_2_11_45_1","DOI":"10.1556\/EuJMI.4.2014.2.7"},{"issue":"4","key":"e_1_2_11_46_1","first-page":"3376","article-title":"Effect of TLR4\/MyD88 signaling pathway on sepsis\u2010associated acute respiratory distress syndrome in rats, via regulation of macrophage activation and inflammatory response","volume":"15","author":"Zhou S","year":"2018","journal-title":"Exp Ther Med"},{"doi-asserted-by":"publisher","key":"e_1_2_11_47_1","DOI":"10.1016\/j.ejccm.2016.10.002"},{"doi-asserted-by":"publisher","key":"e_1_2_11_48_1","DOI":"10.1074\/jbc.273.21.13313"},{"doi-asserted-by":"publisher","key":"e_1_2_11_49_1","DOI":"10.1186\/1476-9255-9-33"},{"doi-asserted-by":"publisher","key":"e_1_2_11_50_1","DOI":"10.1152\/ajpregu.00098.2018"},{"doi-asserted-by":"publisher","key":"e_1_2_11_51_1","DOI":"10.1590\/S0100-879X2007001100014"},{"doi-asserted-by":"publisher","key":"e_1_2_11_52_1","DOI":"10.3109\/08830185.2014.889129"},{"issue":"1","key":"e_1_2_11_53_1","first-page":"35","article-title":"Modulating efficacy of thyme oil in rats exposed to \u03b3\u2010radiation","volume":"6","author":"Nada A","year":"2013","journal-title":"J Radiation Res Appl Sci"}],"container-title":["Biotechnology and Applied Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bab.2580","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T17:32:03Z","timestamp":1723051923000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1002\/bab.2580"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,21]]},"references-count":52,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,8]]}},"alternative-id":["10.1002\/bab.2580"],"URL":"https:\/\/doi.org\/10.1002\/bab.2580","archive":["Portico"],"relation":{},"ISSN":["0885-4513","1470-8744"],"issn-type":[{"type":"print","value":"0885-4513"},{"type":"electronic","value":"1470-8744"}],"subject":[],"published":{"date-parts":[[2024,3,21]]},"assertion":[{"value":"2023-07-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}