{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T19:17:08Z","timestamp":1775675828170,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T00:00:00Z","timestamp":1714435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T00:00:00Z","timestamp":1714435200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Recently, concern has been raised about the spread of human mpox virus, and the demand for rapid detection reagents for mpox virus has increased. This study aims to establish a time\u2010resolved fluorescence immunochromatography (TRFICO) method for qualitative\/quantitative detection of mpox virus. A double\u2010antibody sandwich TRFICO method was optimized and established using mpox recombinant fusion antigen and its paired monoclonal antibody. The test performance of the method was evaluated using mpox fusion antigen and control serum, including sensitivity, linearity range, specificity, precision, and reference interval. We successfully established a TRFICO method for qualitative\/quantitative detection of mpox antigen, its linearity range 0\u2013100\u00a0ng\/mL, analytical sensitivity 0.017\u00a0ng\/mL, and reference intervals greater than 0.045\u00a0ng\/mL. No cross\u2010reaction was detected with various poxvirus and clinical negative controls, with good specificity. All average recoveries of the intra\u2010 and inter\u2010batch ranged from 81.33% to 97.83%, and all\n                    <jats:italic>CVs<\/jats:italic>\n                    were below 10%. Additionally, the TRFICO strips can be stably stored at 37\u00b0C for 7\u00a0days without significant changes in the fluorescence intensity. This TRFICO method, with high sensitivity, linearity range, specificity, precision, and stability with 16\u2010min detection time, provides a new option for qualitative\/quantitative and convenient testing of mpox virus.\n                  <\/jats:p>","DOI":"10.1002\/bab.2594","type":"journal-article","created":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T02:29:25Z","timestamp":1714530565000},"page":"1025-1031","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Quantitative detection of mpox antigen using time\u2010resolved fluorescence immunochromatography"],"prefix":"10.1002","volume":"71","author":[{"given":"Huankun","family":"Liang","sequence":"first","affiliation":[{"name":"Guangzhou Youdi Bio\u2010technology Co., Ltd  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cuicui","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangzhou Youdi Bio\u2010technology Co., Ltd  Guangzhou China"},{"name":"Jinan Laide Bio\u2010technology Co., Ltd  Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiancai","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Laboratory Medicine and Biotechnology Southern Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenqi","family":"Dong","sequence":"additional","affiliation":[{"name":"Guangzhou Zhenda Biopharmaceutical Technology Co., Ltd  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1663-4142","authenticated-orcid":false,"given":"Laiqing","family":"Li","sequence":"additional","affiliation":[{"name":"Guangzhou Youdi Bio\u2010technology Co., Ltd  Guangzhou China"},{"name":"Jinan Laide Bio\u2010technology Co., Ltd  Jinan China"},{"name":"Guangzhou Zhenda Biopharmaceutical Technology Co., Ltd  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,4,30]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciac622"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2019.03.001"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0010141"},{"key":"e_1_2_8_5_1","volume-title":"WHO EPI\u2010WIN webinar: global mpox strategy for elimination and control: open consultation","year":"2023"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-022-01215-4"},{"key":"e_1_2_8_7_1","first-page":"636","article-title":"Serological assay to detect human antibodies against monkey poxvirus","volume":"6","author":"Ren J","year":"2018","journal-title":"Chin J Exp Clin Virol."},{"key":"e_1_2_8_8_1","first-page":"1","article-title":"Establishment of indirect ELISA method for detection of monkeypox virus antibody based on A27L protein","volume":"1","author":"Zhu J","year":"2022","journal-title":"Chin J Vet 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detection of dexamethasone in milk by time\u2010resolved fluorescence immunochromatography","volume":"22","author":"Zhou F","year":"2022","journal-title":"J Food Saf Qual."},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2021.129739"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2022.339833"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.24513"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-021-03612-0"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/s16071094"}],"container-title":["Biotechnology and Applied 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