{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T01:10:37Z","timestamp":1791940237939,"version":"4.3.4"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T00:00:00Z","timestamp":1719792000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Alveolar ridge resorption following tooth extraction poses significant challenges for future dental restorations. This study investigated the efficacy of fish scale\u2013derived hydroxyapatite (FSHA) as a socket preservation graft material to maintain alveolar bone volume and architecture. FSHA was extracted from *<jats:italic>Labeo rohita<\/jats:italic>* fish scales and characterized using Fourier transform infrared (FTIR) analysis. In vitro, biocompatibility and osteogenic potential were assessed using Saos\u20102 human osteosarcoma cells. Cell viability, migration, and proliferation were evaluated using MTT and scratch assays. In vivo performance was assessed in a rat model, and FSHA was compared to a commercial xenograft (Osseograft) and ungrafted controls. Histological analysis was performed at 8\u2010week post\u2010implantation to quantify new bone formation. FTIR confirmed the purity and homogeneity of FSHA. In vitro, FSHA enhanced Saos\u20102 viability, migration, and proliferation compared to controls. In vivo, FSHA demonstrated superior bone regeneration compared to Osseograft and ungrafted sites, with balanced graft resorption and new bone formation. Histological analysis revealed an active incorporation of FSHA into new bone, with minimal gaps and ongoing remodeling. Approximately 50%\u201360% of FSHA was resorbed by 8 weeks, closely matching the rate of new bone deposition. FSHA stimulated more bone formation in the apical socket region than in coronal areas. In conclusion, FSHA is a promising biomaterial for alveolar ridge preservation, exhibiting excellent biocompatibility, osteogenic potential, and balanced resorption. Its ability to promote robust bone regeneration highlights its potential as an effective alternative to currently used graft materials in socket preservation procedures.<\/jats:p>","DOI":"10.1002\/bab.2627","type":"journal-article","created":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T04:49:09Z","timestamp":1719895749000},"page":"1272-1280","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Fish scale\u2013derived hydroxyapatite for alveolar ridge preservation"],"prefix":"10.1002","volume":"71","author":[{"given":"P. M.","family":"Prathibha","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Pushpagiri College of Dental Sciences, Pushpagiri Medical Society  Tiruvalla Kerala India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. G.","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Periodontology Pushpagiri College of Dental Sciences, Pushpagiri Medical Society  Tiruvalla Kerala India"},{"name":"Pushpagiri Research Center Pushpagiri Institute of Medical Sciences and Research Centre, Pushpagiri Medical Society  Tiruvalla Kerala India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7683-3146","authenticated-orcid":false,"given":"Y. B.","family":"Dalvi","sequence":"additional","affiliation":[{"name":"Pushpagiri Research Center Pushpagiri Institute of Medical Sciences and Research Centre, Pushpagiri Medical Society  Tiruvalla Kerala India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. G.","family":"Varghese","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Pushpagiri College of Dental Sciences, Pushpagiri Medical Society  Tiruvalla Kerala India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. K.","family":"Binsi","sequence":"additional","affiliation":[{"name":"ICAR\u2013Central Institute of Fisheries Technology  Cochin India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. A.","family":"Zynudheen","sequence":"additional","affiliation":[{"name":"ICAR\u2013Central Institute of Fisheries Technology  Cochin India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Lekshmi","sequence":"additional","affiliation":[{"name":"Department of Periodontology Pushpagiri College of Dental Sciences, Pushpagiri Medical Society  Tiruvalla Kerala India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6530-9437","authenticated-orcid":false,"given":"J.","family":"Shilpa","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Sethu Institute of Technology  Virudhunagar Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vellappally","family":"Sajith","sequence":"additional","affiliation":[{"name":"Dental Health Department, College of Applied Medical Sciences King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anil","family":"Sukumaran","sequence":"additional","affiliation":[{"name":"Pushpagiri Research Center Pushpagiri Institute of Medical Sciences and Research Centre, Pushpagiri Medical Society  Tiruvalla Kerala India"},{"name":"Oral Health Institute, Department of Dentistry Hamad Medical Corporation  Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40729-022-00456-w"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.5037\/jomr.2019.10102"},{"key":"e_1_2_7_4_1","first-page":"313","article-title":"Bone healing and soft tissue contour changes following single\u2010tooth extraction: a clinical and radiographic 12\u2010month prospective study","volume":"23","author":"Schropp L","year":"2003","journal-title":"Int J Periodontics Restorative Dent"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.21037\/atm-20-1432"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.4103\/njms.njms_224_22"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12469"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma16114117"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.4028\/www.scientific.net\/KEM.587.325"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2018.e00884"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceramint.2011.01.018"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2016.01.051"},{"key":"e_1_2_7_13_1","first-page":"80","article-title":"Hydroxyapatite from fish for bone tissue engineering: a promising approach","volume":"7","author":"Granito RN","year":"2018","journal-title":"Int J Mol Cell Med"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matchar.2016.09.034"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1039\/d3nj03005a"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10973-021-10842-y"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.30"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1590\/1678-7757-2017-0326"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-925X-12-99"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1369\/0022155417733708"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0501.2009.01831.x"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1563\/0.824.1"},{"key":"e_1_2_7_24_1","first-page":"393","article-title":"Ridge preservation utilizing an alloplast prior to implant placement\u2010clinical and histological case reports","volume":"12","author":"Froum S","year":"2000","journal-title":"Pract Periodontics Aesthet Dent"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s41779-022-00813-3"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2007.08.003"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.3c01106"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v019a06"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.4028\/www.scientific.net\/AMR.584.212"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1179\/174367613X13789812714425"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2015.03.020"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.4161\/cam.36224"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10616-015-9895-4"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874210601408010056"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2019.02.067"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scp.2022.100726"},{"key":"e_1_2_7_37_1","first-page":"387","article-title":"A histomorphometric evaluation of factors influencing the healing of bony defects surrounding implants","volume":"20","author":"Pretorius JA","year":"2005","journal-title":"Int J Oral Maxillofac Implants"}],"container-title":["Biotechnology and Applied Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bab.2627","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T04:36:20Z","timestamp":1744346180000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1002\/bab.2627"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7]]},"references-count":36,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["10.1002\/bab.2627"],"URL":"https:\/\/doi.org\/10.1002\/bab.2627","archive":["Portico"],"relation":{},"ISSN":["0885-4513","1470-8744"],"issn-type":[{"value":"0885-4513","type":"print"},{"value":"1470-8744","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7]]},"assertion":[{"value":"2023-11-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}