{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T02:26:48Z","timestamp":1791685608570,"version":"4.3.4"},"reference-count":47,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T00:00:00Z","timestamp":1719273600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In spite of 150 years of studying malaria, the unique features of the malarial parasite,\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    , still perplex researchers. One of the methods by which the parasite manages its gene expression is epigenetic regulation, the champion of which is PfGCN5, an essential enzyme responsible for acetylating histone proteins. PfGCN5 is a \u223c170\u00a0kDa chromatin\u2010remodeling enzyme that harbors the conserved bromodomain and acetyltransferase domain situated in its C\u2010terminus domain. Although the PfGCN5 proteolytic processing is essential for its activity, the specific protease involved in this process still remains elusive. Identification of PfGCN5 interacting proteins through immunoprecipitation (IP) followed by LC\u2010tandem mass spectrometry analysis revealed the presence of food vacuolar proteins, such as the cysteine protease Falcipain 3 (FP3), in addition to the typical members of the PfGCN5 complex. The direct interaction between FP3 and PfGCN5 was further validated by in vitro pull\u2010down assay as well as IP assay. Subsequently, use of cysteine protease inhibitor E64d led to the inhibition of protease\u2010specific processing of PfGCN5 with concomitant enrichment and co\u2010localization of PfGCN5 and FP3 around the food vacuole as evidenced by confocal microscopy as well as electron microscopy. Remarkably, the proteolytic cleavage of the nuclear protein PfGCN5 by food vacuolar protease FP3 is exceptional and atypical in eukaryotic organisms. Targeting the proteolytic processing of GCN5 and the associated protease FP3 could provide a novel approach for drug development aimed at addressing the growing resistance of parasites to current antimalarial drugs.\n                  <\/jats:p>","DOI":"10.1002\/bab.2630","type":"journal-article","created":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T02:09:50Z","timestamp":1719367790000},"page":"1304-1315","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<i>Plasmodium falciparum<\/i>\n                    cysteine protease Falcipain 3: A potential enzyme for proteolytic processing of histone acetyltransferase PfGCN5"],"prefix":"10.1002","volume":"71","author":[{"given":"Poonam","family":"Nagar","sequence":"first","affiliation":[{"name":"Special Centre for Molecular Medicine Jawaharlal Nehru University  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krishanu","family":"Bhowmick","sequence":"additional","affiliation":[{"name":"Special Centre for Molecular Medicine Jawaharlal Nehru University  New Delhi India"},{"name":"The Institute for Bioelectronic Medicine, The Feinstein Institutes for Medical Research Northwell Health  Manhasset New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aishwarya","family":"Chawla","sequence":"additional","affiliation":[{"name":"Special Centre for Molecular Medicine Jawaharlal Nehru University  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Zubbair","family":"Malik","sequence":"additional","affiliation":[{"name":"School of Computational and Integrative Sciences Jawaharlal Nehru University  New Delhi India"},{"name":"Department of Genetics and Bioinformatics Dasman Diabetes Institute  Dasman Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naidu","family":"Subbarao","sequence":"additional","affiliation":[{"name":"School of Computational and Integrative Sciences Jawaharlal Nehru University  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inderjeet","family":"Kaur","sequence":"additional","affiliation":[{"name":"International Centre for Genetic Engineering and Biotechnology  New Delhi India"},{"name":"Department of Biotechnology Central University of Haryana  Mahendergarh Haryana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9732-9456","authenticated-orcid":false,"given":"Suman Kumar","family":"Dhar","sequence":"additional","affiliation":[{"name":"Special Centre for Molecular Medicine Jawaharlal Nehru University  New Delhi India"},{"name":"TERI School of Advanced Studies, Vasant Kunj  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00447382"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.236489"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168\u20109525(03)00115\u2010X"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20445"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.BBRC.2011.01.088"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1600312"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006\u20102952(98)00140\u20103"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.18.10.5659"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.9.5895"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.06.026"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00062\u201007"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.154"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/S11103\u2010009\u20109594\u20107\/FIGURES\/7"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.CMET.2006.04.013"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/JBC.M109.015164"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1583"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2010.125"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006512"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M408123200"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M703316200"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/J.1600\u20100854.2004.00244.X"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1042\/0264\u20106021:3600481"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz1044"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2015.09.005"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/SCIENCE.781840"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4223"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102958.2008.06316.x"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574\u20106968.12343"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/GB\u20102003\u20104\u20109\u2010R60\/TABLES\/3"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/1755\u20108794\u20104\u201026"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2018.03.003"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2022.101713"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009351"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20031487"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M004459200"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1074\/JBC.M310536200"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PONE.0005694"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/345195"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1074\/JBC.M603776200"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb200307147"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20100854.2009.01018.x"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102958.2006.05244.x"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2010189"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1074\/JBC.M310536200"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2007.06.008"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1146\/ANNUREV.BIOCHEM.71.090501.145453"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612023394197"}],"container-title":["Biotechnology and Applied Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bab.2630","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T00:36:04Z","timestamp":1744331764000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1002\/bab.2630"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,25]]},"references-count":47,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["10.1002\/bab.2630"],"URL":"https:\/\/doi.org\/10.1002\/bab.2630","archive":["Portico"],"relation":{},"ISSN":["0885-4513","1470-8744"],"issn-type":[{"value":"0885-4513","type":"print"},{"value":"1470-8744","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,25]]},"assertion":[{"value":"2024-04-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}